2016/10/31 17:36
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Data in this application note were provided by M. Kawakatsu and Y. Hayashi, Applied
Technical Department, M&S Instruments, Inc., Japan.
As the size‐selective separation of nucleic acids is a necessary step in many molecular
biological analyses performed in clinical, forensic, and other types of laboratories, the
development of optimal DNA separation methods is important. Liquid
chromatographic separation techniques involve simple automated sample purification
steps and provide high resolution of DNA molecules, thereby enabling efficient DNA
separation. Furthermore, fragments can be identified and quantified directly when
using liquid chromatography methods.
The separation and characterization of modified DNA is particularly relevant, as
general interest in next‐generation sequencing (NGS) is expanding.1 This application
note describes the successful separation of methylated lambda phage DNA fragments
on a Gilson HPLC system using a Sepax PolyRP‐1000 column. Prior to HPLC analysis, a
gradient method was developed to provide sufficient resolution of the DNA fragments
(25‐2000 bp). These results demonstrate the feasibility of separating DNA fragments,
including those containing methylated modifications, by HPLC. This highly effective
separation method can thus be applied in a wide variety of analyses, including the
preparation steps of next‐generation sequencing workflows.
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NanoBrook产品应用-12-ZetaPALS
文献名: Structural, morphological and optical properties of Bi NPs obtained by laser ablation and their selective detection of L-cysteine 作者: Ramon Gabriel Teixeira Rosaa, Celso de Araujo Duartea , Wido Herwig Schreinera, Ney Pereira Mattoso Filhoa, Arandi Ginane Bezerra Jr.b, Andersson Barisonc, Fernanda Maria Marins Ocamposc a Departamento de Física, Universidade Federal do Paraná Centro Politécnico, CP 19044, 81531-990 Curitiba (PR), Brazil b Departamento Acadêmico de Física, Universidade Tecnológica Federal do Paraná, 80230-901 Curitiba (PR), Brazil c Departamento de Química, Universidade Federal do Paraná Centro Politécnico, CP 19081, 81531-990 Curitiba (PR), Brazil 摘要:In the present work we show the results of the investigation of the properties of bismuth nanoparticles (NPs) obtained by the laser ablation in water. The samples were characterized by various techniques: UV/vis spectroscopy, dynamic light scattering, transmission electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, nuclear magnetic resonance and zeta potential. The NPs were metallic and surface oxidated, exhibiting absorption peak at 257 nm due to the surface plasmon resonance. The interaction between the NPs and various amino acids was studied and revealed a selective sensibility to cysteine on a range of concentration from 80 to 720 μM, which we attribute to a dimerization of cysteine to cystine probably complexed to the nanoparticle surface by electrostatic interactions, leading to the modification of the NP absorption spectrum. 关键词:Bismuth; Nanoparticles; Laser ablation; Cysteine
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