自动化固相萃取法对蜂蜜中氯黴素的确认

This study was performed in collaboration with Curtis Hedman at the WI State
Laboratory of Hygiene, Madison, WI, USA

Chloramphenicol (CAP) is a potent broad‐spectrum antibiotic that can cause
potentially serious side effects in humans. The use of this antibiotic in foods is highly
regulated. Recent reports of honey contaminated with CAP has increased the need
for laboratories to determine the presence of CAP in imported honey samples
(Helle, N. and Gil, C., 2006; McGrath, T., 2006). The presence of CAP in honey is
typically determined by liquid chromatography‐mass spectrometry or LC/MS‐MS
(Quon, D. et al., 2006; Turnipseed, S. et al., 2002; Zhao, L. and Ball, C., 2009). Since
honey is a complex matrix and can cause ion suppression and other matrix
interferences, CAP is typically extracted by liquid/liquid extraction(LLE) followed by
purification using one or two solid phase extraction (SPE) steps prior to analysis.

These steps can be very time‐consuming, labor intensive and subject to poor
reproducibility. The purpose of this study was to develop and evaluate an
automated sample preparation procedure for the determination of CAP in honey.
Three SPE cartridges were compared to determine their suitability for purification
after the liquid/liquid extraction step. LC/MS with Selected Ion Monitoring (SIM)
was utilized to evaluate and compare the overall effectiveness of removing
interferents from honey extracts using the three types of SPE cartridge. CAP
recovery was compared for the 3 brands of SPE cartridge using LC/MS‐MS. SPE for
CAP in honey from published methods (Turnipseed, S. et al., 2002; Zhao, L. and Ball,
C., 2009) was readily amenable to automation.