Peridinin-Chlorophyll-protein(PerCP)
多甲藻黄素-叶绿素-蛋白质 复合物
Quantity Size: 5mg, 10mg
Absorbance: λmax = 482 nm
Emission: λmax = 676 nm
Purity: A482/A280>4.0
FORMULATION: Protein solution in 50 mM Tris-HCl (pH 7.5) and 15 mM sodium azide
Concentration: 20.0 mg/ml (20 ± 0.5 mg/ml)
Storage: Store at 2-8EC in the dark. DO NOT FREEZE.
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Description
Peridinin-chlorophyll-protein complex (PerCP) is isolated from Dinophyceae sp. PerCP has an
extremely high absorbance, a high quantum efficiency, a large Stokes shift and a strong excitation
peak at 482 nm with its maximum emission peak at 677 nm. The protein is commonly used for
fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting
(FACS).
PerCP conjugates may be used alone or with AF488 (or FITC) and R-PE for one- to three-color
analyses with a single-laser flow cytometer equipped with an argon laser emiting at 488 nm. Up to
four-color analyses with low compensation are easily achieved by adding APC-conjugated
antibodies with 633 or 635 nm excitation provided by a dual-laser flow cytometer.
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