E.coli MG1655 百欧博伟质粒载体

                 E.coli MG1655 百欧博伟质粒载体

产品信息：

平台编号：bio-82268

规格：1ml甘油管 赠送一个平板

英文名称：E.coli MG1655 

中文名称：大肠杆菌MG1655

货期：款到1-3天出库

运输：冰袋泡沫盒快递

培养方式：LB,37℃

保存：1ml甘油管，-80℃

注意事项：仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。

基因型：

F- lambda- ilvG- rfb-50 rph-1(NC_000913)

基本信息：

抗性：无

培养基：LB

菌株类别：大肠杆菌

培养条件：37℃，有氧，LB

质粒转化：42℃热激

保存方式：20%甘油，-20℃

基本应用：用于基因克隆

备注：大肠杆菌K12系MG1655为非致病性菌株，该菌株基因组大小为4639221bp。

菌株简介：

K-12 MG1655 是一株 K 系列的大肠杆菌，经检测无 Amp 和 Kan 抗性，可用 LB 培养基，37℃培养，其代谢类型是异养兼性厌氧型，可用于代谢研究、基因编辑等实验。

The original K-12 wild-type strain from the Stanford collection contained both the F plasmid and phage lambda. MG1655 was constructed by curing that strain using acridine orange and UV.This strain was sequenced by the Blattner laboratory because it approximates wild-type E. coli and "has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by means of ultraviolet light and acridine orange, respectively." (Blattner, et al. 1997). The mutations listed in the genotype are present in most K-12 strains and were probably acquired early in the history of the laboratory strain. A frameshift at the end of rph results in decreased pyrE expression and a mild pyrimidine starvation, such that thestrain grows 10 to 15% more slowly in pyrimidine-free medium than in medium containing uracil (Jensen 1993). The ilvG- mutation is a frameshift that knocks out acetohydroxy acid synthase II (Lawther, et al. 1982). The rfb-50 mutation is an IS5 insertion that results in the absence of O-antigen synthesis (Liu and Reeves 1994). MG1655 was derived and named by Mark Guyer from strain W1485, which was derived in Joshua Lederberg's labfrom a stab-culture descendant of the original K-12 isolate. This original E. coli strain K-12 was obtained from astool sample of a diphtheria patient in Palo Alto, CA in 1922 (Bachmann, B., pp. 2460-2488 in Neidhardt et al.1996, Escherichia coli and Salmonella: Cellular and Molecular Biology, ASM Press).MG1655 grows on LB andM9 minimal medium (+ Glucose + 1ug/ml thiamine). 

注意事项：

1、为了您的安全和健康，请穿实验服并戴一次性手套操作。本产品仅可用于实验室研究，不能用于动物，人体以及作为食品添加剂等用途。

2、使用甘油菌时可不完全融解，在甘油菌表面蘸取少量涂布固体琼脂平板即可，也可完全融解后使用，但随着冻融次数的增加，菌株的活力会逐渐下降。

中国微生物菌种查询网可以定制制备常用4代定量微生物菌种（生孢梭菌，大肠埃希氏菌，白色念珠菌，铜绿假单胞菌，黑曲霉等）不同浓度如10E3，10E5,10E6浓度。为了满足更广用户的不同需求，单位代理引进国际知名质控菌种产品生产厂家：法国梅里埃MBL，BIOBALL定量产品，赛默飞REMEL科技定性定量商业产品。精准接种量，即用型使用方便快捷，产品种类齐全并且提供产品COA分析证书。PS：Remel质控微生物；BIOBALL质控微生物；Microbiologis（MBL）质粒微生物，点击有链接详细介绍。欢迎您的咨询！