利用Dualex植物氮平衡指数测量仪研究叶片蒸腾作用

Dualex便携式氮平衡-叶绿素-花青素-黄酮醇测量仪

摘要

对比环境生长条件（体外组织培养、体外驯化、气候室、温室和室外）对Populus × canescens clone 84 K叶片发育、角质层蜡组成和离体叶片蒸腾的影响 进行了调查。我们的结果表明，在不同的生长条件下培养时，角质层蜡的总量增加了10倍以上，而蜡的质量组成没有改变。除了直接从组织培养中获得的表现出快速脱水的植株外，完整但分离的叶片的失水率（残余叶片蒸腾）是恒定的，与生长条件无关，因此与蜡含量的增加无关。由于用分离的Astomotous P.×canescens角质层测量的角质层蒸腾速率与离体叶片的残余叶蒸腾速率相同，我们的结果证实，通过气孔关闭后离体叶片的残余蒸腾速率，可以高精度地预测P.×canescens叶片的角质层蒸腾速率。我们的结果令人信服地表明，P.×canescens角质层中蜡含量增加10倍以上不会导致残余（角质层）蒸腾速率降低。

Increased cuticular wax deposition does not change residual foliar transpiration

Abstract

The effect of contrasting environmental growth conditions (in vitro tissue culture, ex vitro acclimatisation, climate chamber, greenhouse and outdoor) on leaf development, cuticular wax composition, and foliar transpiration of detached leaves of the Populus × canescens clone 84 K were investigated. Our results show that total amounts of cuticular wax increased more than 10-fold when cultivated in different growth conditions, whereas qualitative wax composition did not change. With exception of plants directly taken from tissue culture showing rapid dehydration, rates of water loss (residual foliar transpiration) of intact but detached leaves were constant and independent from growth conditions and thus independent from increasing wax amounts. Since cuticular transpiration measured with isolated astomatous P. × canescens cuticles was identical to residual foliar transpiration rates of detached leaves, our results confirm that cuticular transpiration of P. × canescens leaves can be predicted with high accuracy from residual transpiration of detached leaves after stomatal closure. Our results convincingly show that more than 10-fold increased wax amounts in P. × canescens cuticles do not lead to decreased rates of residual (cuticular) transpiration.

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