北大徐福留：非损伤对蕨类耐镉机制的研究NMT植物重金属创新科研平台

NMT作为生命科学底层核心技术，是建立活体创新科研平台的必备技术。2005年~2020年，NMT已扎根中国15年。2020年，中国NMT销往瑞士苏黎世大学，正式打开欧洲市场。

研究使用平台：NMT植物重金属创新科研平台
期刊：Chemosphere

主题：NMT对蕨类耐镉机制的研究

标题：Influence of Cd exposure on H+ and Cd2+ fluxes in the leaf, stem and root of a novel aquatic hyperaccumulator - Microsorum  pteropus
影响因子：5.108
检测指标：Cd2+、H+流速
检测样品：有翅星蕨叶片、茎、根成熟区

Cd2+、H+流实验处理方法：

有翅星蕨在0，100μM和500μM Cd2+浓度中处理7天
Cd2+、H+流实验测试液成份：
100/500μM CdCl2，0.1 mM KCl and 0.3 mM MES， pH 6.0

作者：北京大学徐福留、兰心宇

中文摘要（谷歌机翻）

蕨类小种已经被证明是潜在的新型水生镉超积累物。

在这项研究中，使用非损伤微测技术（NMT）来观察暴露于Cd下的翼龙不同组织的离子通量。暴露于500  mM Cd的7天后，蕨类植物的根和叶中Cd积累超过1000 mg / kg Cd，茎中Cd积累约600 mg /   kg，这表明该植物具有丰富的Cd富集和抗性能力。

NMT试验发现，镉暴露后，所有组织中H+通量均增加，其中茎，叶和根的增加最大。Cd2t通量在不同组织中表现出不同的积累水平，低水平的Cd暴露导致流入根和叶，而高水平的Cd暴露导致从根流出。在高水平的Cd暴露下，叶片或低水平和高水平的Cd暴露下，茎中均未观察到明显的涌入或流出。

但是，短暂的高水平Cd暴露表明长期Cd2t流入根部，短期Cd2t从茎和叶中流出。蕨类植物的根对Cd富集和抗药性具有更强的调节机制，低水平暴露后出现涌入，高水平暴露后出现外排。当暴露于Cd时，翼龙的茎显示出较少的运输和吸收。低水平的镉暴露导致个别叶片直接从水培溶液中吸收镉。不同的蕨类植物组织表现出不同的镉富集和抗性机制。

Fig. 3. Net Ht fluxes (line charts A-C) and mean Ht flux (bar chart D)  in leaves (A), stems (B), and roots (C) of M. pteropus under 0, 100 and  500 mM Cd exposure. Values followed by different letters are  significantly different (p < 0.05).

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英文摘要

Microsorum pteropus has been proven to be a potential novel aquatic Cd hyperaccumulator.

In  this study, Non-invasive Micro-test Technology (NMT) was used to   observe the ion fluxes of different M. pteropus tissues under Cd   exposure. M. pteropus can hyperaccumulate more than 1000 mg/kg Cd in   roots and leaves and approximately 600 mg/kg Cd in stems after seven   days of exposure to 500 mM Cd, showing that this plant have a great   capacity for Cd enrichment and resistance.

The NMT test found  H+ fluxes increased in all tissues after Cd exposure, with the largest  increases being observed in stems, followed by the leaves and roots.  Cd2t fluxes showed different accumulation levels in different tissues,  with low-level Cd exposure leading to influxes into roots and leaves,  and high level Cd exposure resulting in effluxes from roots. No  significant influxes or effluxes were observed in leaves under  high-level Cd exposure, or in stems under low- and high-levels of Cd  exposure.

However, transient high-level Cd exposure showed  long-term Cd2t influxes into roots and short-term Cd2t effluxes out of  stems and leaves. The roots of M. pteropus had greater regulation  mechanisms for Cd enrichment and resistance, with influxes occurring  following low-level exposure and effluxes occurring from high-level  exposure. When exposed to Cd, M. pteropus stems showed less  transportation and absorption. Low-level Cd exposure resulted in  individual leaves directly absorbing Cd from hydroponic solutions.  Different Cd enrichment and resistance mechanisms were exhibited by  different M. pteropus tissues.