NMT作为生命科学底层核心技术,是建立活体创新科研平台的必备技术。2005年~2020年,NMT已扎根中国15年。2020年,中国NMT销往瑞士苏黎世大学,正式打开欧洲市场。
研究使用平台:NMT林木耐旱创新科研平台
期刊:New Phytologist
主题:转录因子PdNF-YB21调控杨树根系生长并影响抗旱性
标题:The root-specific NF-Y family transcription factor, PdNF-YB21, positively regulates root growth and drought resistance by ABA-mediated IAA transport in Populus
影响因子:7.299
检测指标:H+
检测样品:杨树根
H+流实验处理方法:
杨树茎段在Hoagland营养液/Hoagland营养液+0.1μM ABA/Hoagland营养液+10μM FLU培养30天后检测根。
H+流实验测试液成份:未写明
作者:北京林业大学夏新莉、周扬颜
中文摘要(谷歌机翻)
根系生长控制在植物对干旱胁迫的适应中起着重要作用,但是这种控制的潜在分子机制仍然很难捉摸。
在此,从胡杨中分离出根特异性核因子Y(NF-Y)转录因子PdNF-YB21。PdNF-YB21的功能机制通过各种形态,生理,分子,生化和光谱学技术进行了表征。
杨树中PdNF-YB21的过表达促进木质部血管高度木质化和扩大的根系生长,从而增加了抗旱性。相反,CRISPR /
Cas9介导的杨树突变体nf-yb21表现出降低的根系生长和抗旱性。PdNF-YB21与B3域转录因子PdFUSCA3(PdFUS3)相互作用。PdFUS3直接激活ABA合成关键基因PdNCED3的启动子,导致水分缺乏的杨树根ABA含量显着增加。杨NF-YB21和FUS3的共表达显着增强了PdNCED3的表达。
此外,ABA促进了IAA在根尖的转运,最终增加了根的生长和抗旱性。
英文摘要
Root growth control plays an important role in plant
adaptation to drought stress, but the underlying molecular mechanisms
of this control remain largely elusive.
Here, a root-specific nuclear factor Y (NF-Y) transcription factor
PdNF-YB21 was isolated from Populus. The functional mechanism of
PdNF-YB21 was characterized by various morphological, physiological,
molecular, biochemical, and spectroscopy techniques.
Overexpression of PdNF-YB21 in poplar promoted root growth with highly
lignified and enlarged xylem vessels, resulting in increased drought
resistance. In contrast, CRISPR/Cas9-mediated poplar mutant nf-yb21
exhibited reduced root growth and drought resistance. PdNF-YB21
interacted with PdFUSCA3 (PdFUS3), a B3 domain transcription factor.
PdFUS3 directly activated the promoter of the ABA synthesis key gene
PdNCED3, resulting in a significant increase in root ABA content in
poplars subjected to water deficit. Coexpression of poplar NF-YB21 and
FUS3 significantly enhanced the expression of PdNCED3.
Furthermore, ABA promoted IAA transport in root tips, which ultimately increased root growth and drought resistance.
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