NMT历史上的今天丨Plant J：拟南芥WRKY8与VQ9调节盐胁迫耐受性

2006~2020，NMT已扎根中国15年。2020年，中国NMT销往瑞士苏黎世大学，正式打开欧洲市场。

NMT历史上的今天

2013年03月02日，中科院西双版纳热带植物园余迪求、胡彦如用NMT在Plant Journal上发表了标题为Arabidopsis  transcription factor WRKY8 functions antagonistically with its   interacting partner VQ9 to modulate salinity stress tolerance的研究成果。

期刊：Plant Journal
主题：拟南芥WRKY8与VQ9调节盐胁迫耐受性

标题：Arabidopsis transcription factor WRKY8 functions   antagonistically with its interacting partner VQ9 to modulate salinity   stress tolerance

影响因子：6.582

检测指标：K+流速

作者：中科院西双版纳热带植物园余迪求、胡彦如

点击观看 访谈：Plant Biotechnol J抗盐文章NMT思路

英文摘要

The WRKY transcription factors have been demonstrated to  play crucial roles in regulating stress responses; however, the exact mechanisms underlying their involvement in stress responses are not   fully understood.

Arabidopsis WRKY8 was predominantly expressed  in roots and was highly upregulated by salt treatment. Disruption of  WRKY8 rendered plants hypersensitive to salt, showing delayed  germination, inhibited post‐germination development and accelerated  chlorosis. Further investigation revealed that WRKY8 interacted with  VQ9, and their interaction decreased the DNA‐binding activity of WRKY8.

The  VQ9 protein was exclusively localized in the nucleus, and VQ9   expression was strongly responsive to NaCl treatment. Mutation of VQ9   enhanced tolerance to salt stress, indicating that VQ9 acts   antagonistically with WRKY8 to mediate responses to salt stress. The   antagonist functions of WRKY8 and VQ9 were consistent with an increased or reduced Na+/K+ concentration ratio, as well as contrasting expression  patterns of downstream stress‐responsive genes in salt‐stressed wrky8  and vq9 mutants.

Moreover, chromatin immunoprecipitation (ChIP)  assays showed that WRKY8 directly bound the promoter of RD29A under salt  conditions. These results provided strong evidence that the VQ9 protein  acts as a repressor of the WRKY8 factor to maintain an appropriate  balance of WRKY8‐mediated signaling pathways to establish salinity  stress tolerance.

中文摘要（谷歌机翻）

WRKY转录因子已被证明在调节压力反应中起关键作用。然而，它们参与应激反应的确切机制尚不完全清楚。

拟南芥WRKY8主要在根中表达，并通过盐处理高度上调。WRKY8的破坏使植物对盐高度敏感，表现出延迟的发芽，抑制了发芽后的发育并加速了萎黄病。进一步的研究表明，WRKY8与VQ9相互作用，并且它们的相互作用降低了WRKY8的DNA结合活性。

VQ9蛋白专门位于细胞核中，并且VQ9表达对NaCl处理有强烈反应。VQ9突变增强了对盐胁迫的耐受性，表明VQ9与WRKY8拮抗，介导对盐胁迫的响应。WRKY8和VQ9的拮抗剂功能与Na+  / K+浓度比的增加或降低，以及盐胁迫的wrky8和vq9突变体中下游胁迫反应基因的相反表达模式一致。

此外，染色质免疫沉淀（ChIP）分析表明，WRKY8在盐条件下直接结合RD29A的启动子。这些结果提供了有力的证据，表明VQ9蛋白可作为WRKY8因子的阻遏物，以维持WRKY8介导的信号通路的适当平衡，从而建立盐度胁迫耐受性。

(c) Net K+ ef?ux in root tips. Seeds were germinated on MS agar medium for 4 days in a vertical manner. The net  immediate K+ ef?ux was measured using the non-injuring technique after  the addition of salt. The insert shows the mean ef?ux rates within the  measuring period of 0–20 min.