NMT历史上的今天丨New Phytol ABA促进拟南芥/水稻适应水胁迫文章发表

旭月公司

2019/09/17 10:26

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NMT历史上的今天

2012年9月16日，中科院南京土壤研究所施卫明、张建华、许卫锋利用NMT在New Phytologist上发表了标题为Abscisic acid accumulation modulates auxin transport in the root tip to enhance proton secretion for maintaining root growth under moderate water stress的研究成果。

2019.09.16 Absci-01.png

期刊：New Phytologist

主题：脱落酸积累调节根尖中的生长素运输以增强质子分泌以在中度水胁迫下维持根生长

标题：Abscisic acid accumulation modulates auxin transport in the root tip to enhance proton secretion for maintaining root growth under moderate water stress

影响因子：6.645

检测指标：H⁺流速

检测部位：拟南芥、水稻根尖（从根冠分界处50、100、200、300、400、500、600、700、800、900、1000、1200、1500、1800、2000、2500、3000、3500、4000和5000μm）

H⁺流实验处理方法：

15日龄水稻幼苗，5％PEG 8000或0.1 μM ABA处理24小时

H⁺流实验测试液成份：文献无

推荐测试液：0.1mM CaCl20.3mM MES，pH6.0

作者：中科院南京土壤研究所施卫明、张建华、许卫锋

英文摘要

Maintenance of root growth is essential for plant adaptation to soil drying. Here, we tested the hypothesis that auxin transport is involved in mediating ABA's modulation by activating proton secretion in the root tip to maintain root growth under moderate water stress.

Rice and Arabidopsis plants were raised under a hydroponic system and subjected to moderate water stress (?0.47 MPa) with polyethylene glycol (PEG). ABA accumulation, auxin transport and plasma membrane H+‐ATPase activity at the root tip were monitored in addition to the primary root elongation and root hair density.

We found that moderate water stress increases ABA accumulation and auxin transport in the root apex. Additionally, ABA modulation is involved in the regulation of auxin transport in the root tip. The transported auxin activates the plasma membrane H+‐ATPase to release more protons along the root tip in its adaption to moderate water stress. The proton secretion in the root tip is essential in maintaining or promoting primary root elongation and root hair development under moderate water stress.

These results suggest that ABA accumulation modulates auxin transport in the root tip, which enhances proton secretion for maintaining root growth under moderate water stress.

中文摘要

保持根系生长对植物适应土壤干燥至关重要。在这里，我们测试了生长素转运通过激活根尖中的质子分泌来介导ABA调节以在中等水分胁迫下维持根生长的假设。

在水培系统下培养水稻和拟南芥植物，并用聚乙二醇（PEG）进行适度的水分胁迫（-0.47MPa）。除了初生根伸长和根毛密度之外，还监测根尖处的ABA积累，生长素转运和质膜H+-ATP酶活性。

我们发现，适度的水分胁迫会增加根尖中ABA的积累和生长素的转运。另外，ABA调节涉及根尖中生长素运输的调节。运输的生长素激活质膜H+-ATPase以沿着根尖释放更多的质子，以适应中度水分胁迫。根尖中的质子分泌对于在中度水分胁迫下维持或促进初生根伸长和根毛发育是必不可少的。

这些结果表明，ABA积累调节根尖中的生长素运输，这增强了质子分泌，从而在中度水分胁迫下维持根生长。

2019.09.16 Absci-02.png

Fig. 1 Effects of moderate water stress or exogenous ABA treatment on H⁺ ?ux along the root tip of rice or Arabidopsis plants (0–5000 lm from the root cap junction). Fifteen-day-old rice or Arabidopsis plants were exposed to control conditions (●, control), moderate water stress (○,5%PEG 8000) or exogenous ABA (▼, 0.1 lM) for 24 h in hydroponics. The values are the means and SD of six replicates from two independent experiments.