FOOD CHEM：南京农大丨钙参与盐胁迫下作物酚类积累的GABA信号转导（钙信号，附NMT实验体系）

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期刊：Food Chemistry

主题：钙参与盐胁迫下作物酚类积累的GABA信号转导（钙信号）

标题：Ca2+ involved in GABA signal transduction for phenolics accumulation in germinated hulless barley under NaCl stress

影响因子：5.399

检测指标：Ca2+流速

检测部位：根尖细胞

Ca2+流实验处理方法：

三日龄的大麦幼苗，60mM NaCl、60mM NaCl + 0.5mM GABA、60mM NaCl + 0.1mM 3-MP分别处理四天和六天

Ca2+流实验测试液成份：

0.1mM KCl, 0.1mM CaCl2, 0.1mM MgCl2, 0.5mM NaCl, 0.3mM MES, 0.2mM Na2SO4，ph 6.0

通讯作者：南京农业大学杨润强、马燕

英文摘要

In this study, in order to investigate the role of Ca2+ in GABA signal transduction involved in phenolics accumulation in barley seedlings under NaCl stress, the seedlings were treated with exogenous GABA and its synthesis inhibitor, 3-mercaplopropionic acid (3-MP), as well as Ca2+ channel blockers La3+, Ca2+ chelator EGTA, and Ca2+ release channel inhibitor 2-aminoethoxydiphenyl borate (2-APB).

The results showed that GABA significantly enhanced phenolics, calcium and calmodulin content. It also induced Ca2+ influx in barley root tips cells, and altered the distribution of Ca2+, making calcium precipitates more uniform and intensive. While, 3-MP treatment led to opposite changes, which suggested that GABA was essential for calcium content increase. In addition, accumulation of phenolics was inhibited by LaCl3, EGTA and 2-APB treatments, and this inhibition could be alleviated partly by exogenous GABA.

Taken together, Ca2+ was involved in GABA signal transduction for phenolics accumulation in barley seedlings under NaCl stress.

 中文摘要（谷歌机翻）

在本研究中，为了研究Ca2+在NaCl胁迫下大麦幼苗中酚类物质积累的GABA信号转导中的作用，用外源GABA及其合成抑制剂3-巯基丙酸（3-MP）处理幼苗。以及Ca2+通道阻滞剂La3+，Ca2+螯合剂EGTA和Ca2+释放通道抑制剂2-氨基乙氧基二苯基硼酸盐（2-APB）。

结果表明，GABA显着增强了酚类，钙和钙调蛋白的含量。它还诱导大麦根尖细胞内的Ca2+流入，并改变Ca2+的分布，使钙沉淀更均匀和密集。然而，3-MP处理导致相反的变化，这表明GABA对钙含量的增加至关重要。此外，LaCl3，EGTA和2-APB处理抑制了酚类物质的积累，这种抑制作用可以通过外源性GABA部分缓解。

总之，Ca2+参与了NaCl胁迫下大麦幼苗中酚类物质积累的GABA信号转导。

结果表明，对照大麦幼苗根尖Ca2+外排，与对照相比，NaCl处理引起的Ca2+由外排向吸收转变。GABA处理得到稳定的Ca2+吸收，其中处理6天的吸收明显高于处理4天吸收。GAD抑制剂（3-MP）在不同处理时期产生了不同的趋势，与单独使用NaCl处理相比，第4天Ca2+的外排速率显着增加，但第6天Ca2+流入量较弱。
注：CK表示大麦用蒸馏水喷洒; N表示60mM NaCl处理; NG表示60mM NaCl + 0.5mM GABA处理; NM表示60mM NaCl + 0.1mM 3-MP处理。