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GALNTL4蛋白抗体
产品编号 LM16227R
英文名称 GALNTL4
中文名称 GALNTL4蛋白抗体
别 名 GalNAc T like protein 4; GalNAc T15; GLT18_HUMAN; GalNAc transferase 18; GALNT15; GALNT18; N acetylgalactosaminyltransferase 15 UDP N acetyl alpha D galactosamine:polypeptide; N acetylgalactosaminyltransferase like 4; N acetylgalactosaminyltransferase like protein 4 UDP N acetyl alpha D galactosamine:polypeptide; Polypeptide GalNAc transferase like protein 4; pp GaNTase like protein 4; Protein UDP acetylgalactosaminyltransferase like protein 4; Protein UDP acetylgalactosaminyltransferase like protein 4 UDP GalNAc:polypeptide; Putative polypeptide N acetylgalactosaminyltransferase like protein 4; UDP GalNAc:polypeptide N acetylgalactosaminyltransferase like protein 4.
研究领域 细胞生物 免疫学 细胞类型标志物
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep,
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 70kDa
细胞定位 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GALNTL4:1-100/607
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 The UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes are substrate-specific proteins that catalyze the transfer of GalNAc (N-acetylgalactosaminyl) to serine and threonine residues onto various proteins, thereby initiating mucin-type O-linked glycosylation in Golgi apparatus. GalNAc-TL4, also known as LGALS14 or polypeptide GalNAc transferase-like protein 4, is a 607 amino acid single-pass type II membrane protein belonging to the glycosyltransferase 2 family and GalNAc-T subfamily. Localizing to Golgi apparatus, GalNAc-TL4 utilizes manganese and calcium as cofactors and may assist with the transfer of N-acetyl-D-galactosamine to a serine or threonine residue on protein receptors. GalNAc-TL4 likely catalyzes the initial reaction in O-linked oligosaccharide biosynthesis and contains a ricin B-type lectin domain, which binds to GalNAc and contributes to glycopeptide specificity, and two conserved domains located in the glycosyltransferase region. The N-terminal domain, also known as domain A or GT1 motif, may be involved in manganese coordination and substrate binding while the C-terminal domain, also known as domain B or Gal/GalNAc-T motif, is likely involved in catalytic reaction and UDP-Gal binding. GalNAc-TL4 exists as two alternatively spliced isoforms.
抗原与抗体的关系
①抗体和相应的抗原结合后,可以促进白细胞的吞噬作用而将抗原消除,使抗原失去致病作用。
②一种抗体只能抵抗一种抗原,而且一种抗体只能受相应的抗原刺激后才能形成。
免疫的功能:
免疫具有三方面的功能:
①防御功能:抵抗抗原的侵入、防止疾病的产生。
②自我稳定:清除体内衰老、死亡和损伤的细胞。
③免疫监视:监视、识别和清除体内产生的异常细胞。
特别提醒:过敏反应:当人体抵抗抗原侵入的功能过强时,在过敏原(引起过敏反应的物质,如某些食物、药物)的刺激下,人体就会发生过敏反应。
1.抗体名称容易出错
抗体一般以抗原来命名,名称核对时最简单也最容易犯错,有些蛋白质有不同亚基(HIF-1α、HIF-1β);有些蛋白质有不同磷酸化状态,例如EGFR和p-EGFR的区别,等等。
除了蛋白质的名称外,有其他后缀。
2.多抗和单抗不同
单抗上面介绍过了,而多抗(polyclonal antibodies)一般用兔子、山羊等大型动物,因为多抗是直接从血液中纯化提取出来的,而不像单抗是用细胞培养出来的。
单克隆抗体的优点是产量持续稳定、特异性较高、但灵敏度不如多抗。如果对抗体的特异性要求高,用量较大或需要长期使用一致的抗体,制备的抗体应用要求多(WB/IP/IF/ICC等),可以选择单克隆抗体。
多抗的特异性较差,存在批间差(因为兔子的血被放光挂掉了,而山羊可以常年养着,放点血出来,在养几天再放血,好残忍),易造成背景,例如在WB中有杂带,在IHC中背景较深等等。但由于多抗识别多个抗原表位,对于丰度偏低的蛋白也更容易检出。