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血小板内皮细胞黏附分子1抗体
产品编号 LM0195R
英文名称 CD31
中文名称 血小板内皮细胞黏附分子1抗体
别 名 platelet endothelial cell adhesion molecule precursor-1; PECAM-1; PECAM1; Adhesion molecule; CD31 antigen; CD31 EndoCAM; Endocam; FLJ34100; FLJ58394; GPIIA; Pecam 1; PECA1_HUMAN; PECAM 1 CD31 EndoCAM; PECA1; Pecam1; Platelet endothelial cell adhesion molecule; Platelet/endothelial cell adhesion molecule 1; Adhesion molecule; Platelet/endothelial cell adhesion molecule.
研究领域 肿瘤 心血管 细胞生物 免疫学 信号转导 干细胞 细胞粘附分子 细胞表面分子 糖蛋白 细胞类型标志物 血管内皮细胞 t-淋巴细胞 内皮细胞
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
产品应用 WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 78kDa
细胞定位 细胞膜
性 状 Lyophilized or Liquid
浓 度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD31:681-738/738 <Cytoplasmic>
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Type I membrane protein. SIZE: 738 amino acids; 82536 Da. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart. PTM: Phosphorylated on Ser and Tyr residues after cellular activation. SIMILARITY: Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD31 is implicated in several functions, including transendothelial migration of leukocytes, angiogenesis, and integrin activation. Tyr-690 plays a critical role in leukocyte transendothelial migration (TEM) and is required for efficient trafficking of CD31 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. CD31 prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of CD31 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of CD31 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
血小板内皮细胞黏附分子-1 ( Platelet/ endothelial cell adhesion molecule, PECAM-1)在血小板、内皮细胞、单核细胞、嗜中性细胞及某些T细胞亚群上表达的质膜糖蛋白,属于免疫球蛋白超基因家族成员,在细胞外结构域中有6个C2亚类免疫球蛋白样保守性同原单位。在炎症应答中起作用。
抗原抗体结合特点
1)特异性:抗原分子只能与由它刺激所产生的抗体结合而起反应的专一性能;
2)可逆性:是指Ag与相应Ab结合成IC后,在一定条件下可解离为游离抗原与抗体。
3)阶段性:
一阶段:特异性结合阶段 (①反应速度快,几秒钟或几分钟即可完成 ②不出现肉眼可见反应);
第二阶段:可见阶段(表现为凝集、沉淀、补体结合等反应 ①反应进行慢,需要几分钟、几十分钟或更长②受电解质、温度、酸碱度等多种因素影响)。
4)比例性:(最适比或等价点)是指抗原与抗体发生可见反应需遵循一定的量比关系,只有当二者浓度比例适当时,才出现可见反应。
抗原抗体反应影响因素:
(一)反应物自身的因素
5) 抗体:不同来源的抗体,反应性各有差异,抗体的浓度、特异性和亲和力都影响抗体抗原反应,为提高试验的可靠性,应选择高特异性、高亲和力的抗体作诊断试剂.等价带的宽窄也影响抗原抗体复合物的形成,单克隆抗体不适用于沉淀反应.
6) 抗原:抗原的理化性状、分子量、抗原决定簇的种类及数目均可影响反应结果.颗粒性抗原出现凝集反应,可溶性抗原出现沉淀反应,单价抗原与相应抗体结合不出现沉淀现象.
(二)反应环境条件
7) 酸碱度:抗原抗体反应必须在合适的pH环境中进行.蛋白质具有两性电离性质,因此每种蛋白质都有固定的等电点.抗原抗体反应一般在pH6~9进行,有补体参与的反应pH为7.7.4,pH过高或过低都将影响抗原与抗体反应.
8) 温度:在一定范围内,温度升高可加速分子运动,抗原与抗体碰撞机会增多,使反应加速.一般为15℃~40℃,常用的抗原抗体反应温度为37℃,温度如高于56℃,可导致已结合的抗原抗体再解离,甚至变性或破坏.每种试验都有其独特的最适反应温度要求.此外,适当振荡也可促进抗原抗体分子的接触,加速反应.
9) 电解质:抗原与抗体发生特异性结合后,虽由亲水胶体变为疏水胶体,若溶液中无电解质参加,仍不出现可见反应.为了促成沉淀物或凝集物的形成,常用0.85%NaCl或各种缓冲液作为抗原及抗体的稀释液.