人体血液循环模拟系统

人体血液循环模拟系统，VenaFlux 微流体细胞研究系统 Automated microfluidic platform是提供在活细胞水平上微流体活性筛选的完整解决方案。操作简单，分析效率高，可大大提高药品的开发和筛选效率。

VenaFlux 系统是第一个能进行连续流体细胞分析的半自动化微流体分析系统。配合使用Cellix生物芯片和细胞分析软件，VenaFlux 系统可检测粘附到微毛细管上的细胞，微毛细管中覆盖抗体或者培养有内皮细胞，模拟生理流动产生一定的剪切力。

VenaFlux 系统操作简单，可减少耗时耗钱的动物模型实验，获得特异性强，准确性高，重复性好的结果。
 

VenaFlux流体细胞分析仪产品特点：
 

广泛使用于各种细胞悬浮液；

生物芯片可选择用光学显微镜做细节研究；

微流体泵可方便的控制流体剪切力在0.05-200 Dyne/cm2范围内；

在分析中可调整剪切力；

流体速度可控制在pL/min到mL/min；

可做nL到mL 的样品分析；

使用DucoCell 分析软件（DucoCell Analysis Software），可简单快速的调节好显微镜的各项参数；

统计分析生成的图表（如：细胞粘附性VS.剪切力）可方便的导入Excel文档；

电子机械臂移动生物芯片，清洗细微组件和稀释细胞样品；

VenaFlux系统的软件同步调节泵，翻译阶段筛选，图像采集，样品移动和流体控制，各项操作界面均是用户友好型，系统先进又易于操作；

主要配置方案：
 

一、Partial Platform -STARTER-1.0：

      1、Mirus Nanopump 1.0

      2、1 pack VenaEC biochips

      3、1 pack Vena8 biochips

      4、Frame for VenaEC biochips

      5、DucoCell Software ver 1.1
 

二、Microfluidic SP1.0 (Basic full Package)-MICROSP-1.0：

      1、arl Zeiss Axiovert 40 microscope, CCD Deltapix DP200 camera, Dell PC, Microscope cage incubator      

      2、Mirus Nanopump 1.0 inc. VenaFluxAssay software   

      3、1 pack Vena8 biochips   

      4、 1 pack VenaEC biochips   

      5、DucoCell Software ver 1.1 
 

三、CMicrofluidic SP1.1-MICROSP-1.1：

      1、Carl Zeiss Axiovert 40 CFL microscope, CCD Deltapix DP450 camera, Dell PC, Microscope cage incubator, Fluorescent and brightfield shutters ，  

      2、Mirus Nanopump 1.0 inc. VenaFluxAssay software   

      3、1 pack Vena8 biochips   

      4、1 pack VenaEC biochips   

      5、DucoCell Software ver 1.1 
 

四、Microfluidic SP1.2-MICROSP-1.2:

      1、Carl Zeiss Axiovert 40 CFL microscope, CCD Deltapix DP450 camera, Dell PC, Microscope cage incubator, Fluorescent and brightfield shutters,motorised

stage   

      2、Mirus Nanopump 1.0 inc. VenaFluxAssay software   

      3、1 pack VenaEC biochips   

      4、1 pack Vena8 biochips   

      5、DucoCell Software ver 1.1 
 

五、VenaFlux-VENAFLUX-1.0：

      1、Carl Zeiss Axiovert 40 CFL microscope, CCD Deltapix DP450 camera, Dell PC, Microscope cage incubator, Fluorescent and brightfield shutters   

      2、VenaFluxAssay software plus automatic image capture   

      3、Robotic sample handler   

      4、Mirus Nanopump 1.1 inc. 8-way Manifold   

      5、1 pack VenaEC biochips   

      6、1 pack Vena8 biochips   

      7、Frame for VenaEC biochips   

      8、DucoCell Software ver 1.1

主要技术参数：
 

Microscope Axiovert 40:

Contrast option: Brightfield, Phase, Varel.

Objectives: 10x LD A-Plan, 20x LD A-Plan, 32x CP-Achromat, 40x LD A-Plan

Eyepieces: E-PL 10x/20 Br., 10x/20 Br. foc.

Stage: Vena8 biochip compatible stage holder, Eppendorf tubes holder.

Mirus 1.0 Nanopump

Specifications: Shear Stress Range of 0.05–20 dyne/cm2, 

Volumetric Flow Rates 100nL–20μL/minute (100μL syringe), 

Sample Volume Increments Freely adjustable Linear Velocity Range of 10 μm/s to 10 cm/s; 

Flow Direction Reversible;

Sample Volume Aspiration Accuracy ±1% ; 

Shear Stress Accuracy ±0.5%; 

Sample Volume Aspiration Precision < 1%.

Multichannel: 16-Way manifold allowing individual addressing microchannels of Vena8 biochip.

Microscope Cage incubator:

Capabilities: Temperature control ± 0.10C, CO2 and humidity module.

Options: Temperature monitoring software module, black panels for use in fluorescent microscopy.

Deltapix DP 200 USB 2.0 camera

Specifications: 1.3 Mega Pixels (1,280 x 1,024), Color CDD, 1/2" format, Pixel 5.2 μm x 5.2 μm, 

Resolution: 15 FPS @ 1,280 x 1,024 pixels; 24 FPS @ 1,204 x 768 pixels; 37 FPS @ 800 x 600 pixels; 60 FPS @ 640 x 480 pixels. Dynamic Range > 60 dB.

Dell Optiplex 745 PC computer

Configuration: Intel Core2 processor; 1.86 GHz; 2GB RAM; ATI Radeon X1300PRO; 

Objective Imaging stage control hardware; 19” LCD monitor. 

Windows XP Professional SP2; 

DeltaPix Viewer Software; 

FlowAssay and DucoCell software preinstalled.

FlowAssay Software.

Capabilities: Execution of preset protocols. 

Intuitive stepwise protocol interface with steps hierarchy and status control.

DucoCell software

Specifications: Automatic Counting and Detection; 

Morphological parameters analyzed automatically(Area, diameter, form-factor, ellipticity, eccentricity, orientation, perimeter, asymmetry, cell centroid coordinates, elliptical axis); 

Cell sorting and filtering; 

Ability to analyze sub-populations based on inclusion/exclusion of morphological parameters. 

Data may be exported to Excel spreadsheet incorporating automatic graph generation (e.g. % cell adhesion vs. shear stress). 

File Formats analyzed*.jpg, *.jpeg, *.tif, *.tiff, *.bmp, *.gif, *.png.

Vena8 biochips pack of 10

Specifications: Minimum Sample Volume 3 μL; 

Maximum Sample Volume 100 μL; Shear Stress Range of 0.05– 20 dyne/cm2, steps of 0.05dyne/cm2. 

Number of channels per biochip is 8; 

Volume of each channel is 0.8μL. 

Dead volume at input port is 0.1 μL. Vena8 biochip dimensions are 400 μm (W) x 100 μm (D) x 20 mm(L).

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