VENA8 ENDOTHELIAL+微流体芯片

VENA8 ENDOTHELIAL+微流体芯片的主要特点：

用于细胞滚动、粘附分子研究或细胞间相互作用研究

适用于各种细胞悬浮液(原代和细胞株)包括T细胞、单核细胞、外周血单个核细胞、中性粒细胞、嗜酸性粒细胞、血小板和全血(肝素化)。

Vena8荧光+ TM和 Vena8TM：可以用标准移液器将一系列的粘附因子包覆毛细管外壁。不同的黏附分子包括VCAM(血管细胞粘附因子),ICAM(细胞间粘附因子),MAdCAM，纤维连接蛋白，vWF(人血管性血友病因子/瑞斯托霉素辅因子)、胶原蛋白、纤维蛋白原等。

Vena8内皮细胞+ TM和VenaECTM：容易种植和培养各种内皮细胞(层)包括HUVEC(人脐静脉血管内皮细胞)，HMVEC(人微血管内皮细胞)，HCAECs(人冠状动脉内皮细胞)等等。

生物芯片具有良好的光学特性，可在显微镜下进行清晰的观察和进一步的研究。

剪切应力范围：0.05-20 dyne/ cm2，可以通过MirusTM Nanopump精确控制。

剪切应力的大小及连续变化的参数可以设置。

可在流动状态下实时成像。

The Vena8 Endothelial+™ biochip is ideal for cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow.  Ideal for use with PCR.  Compatible with brightfield, phase contrast imaging and fluorescence microscopy.

Features:

• Cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow.  

• Cells cultured within the biochip channels may be retrieved post-assay for subsequent studies (up to 320,000 cells); e.g. PCR.  Substrate is removed from biochip and cells are scraped off.

• Suitable for whole blood, primary cells, rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.

• No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.

• No luer lock connections which increase dead volume.  Cellix's biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable.  Dead volume is at input port ~ 0.1μL.

Applications:  Flow assays using minimal sample volumes (whole blood, cell suspensions, proteins etc.); brightfield, phase contrast and fluorescence microscopy.

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