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大鼠小鼠儿茶酚胺遥测系统采用快速扫描循环伏安法(FSCV)的工作原理，专门用于儿茶酚胺(i.e., dopamine, norepinephrine, and serotonin)的测量。可以用于测量自由清醒活动状态下的动物。该遥测系统适用于大鼠。

FAST SCAN CYCLIC VOLTAMMETRY SYSTEM

Pinnacle’s FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM is specifically designed to simplify the measurement of catecholamines (i.e., dopamine, norepinephrine, and serotonin). Pinnacle offers turn-key systems for both mice and rats. Both the wireless rat system and the tethered mouse system use carbon fiber sensors to enable short-term measurement in the brains of freely moving animals.

 

HOW IT WORKS

Biogenic amine levels are detected by rapidly cycling a voltage across an implanted carbon fiber sensor and measuring the resultant current. Our systems can measure spontaneous sub-second neurotransmitter release events while conducting detailed behavioral studies. Both the wireless and tethered systems sweep from 250 to 400 V/s in a user-selectable range spanning -1.1 to +1.3 V. All systems have built-in support for controlling an external stimulus.
l  Voltage Span: -1.1 V to +1.3 V
l  Range: 250 – 400 V/s 
l Sweeps/second: 5 - 10 
l Points/sweep: 1000 

 

Analysis

Dopamine (blue), serotonin (green), and norepinephrine (pink) have specific voltammogram profiles when detected by FSCV.
 
The WIRELESS FAST SCAN CYCLIC VOLTAMMETRY (FSCV) SYSTEM for rats uses Bluetooth® to transmit data. The system resides in a headmounted enclosure and wirelessly transmits data to a computer. The battery is easily accessible and readily exchangeable in real-time to support extended recordings. The system is ideal for mazes and enclosed environments, such as metabolic and behavioral chambers. It comes with Pinnacle’s FREE 8500 software.
The Rat Hat houses the battery and protects the system.
2. A low-powered, wireless FSCV board transmits digitized signals to a paired Bluetooth USB dongle.
The Rat Hat bottom contains the electronics, guide cannula, and carbon fiber sensor. It is affixed to the skull with bone screws and dental acrylic. Stereotaxically placed guide cannulas allow easy insertion of carbon fiber sensors.
 

CARBON FIBER

Pinnacle’s FSCV system uses CARBON FIBER SENSORS (CFSs) to measure the presence of dopamine and other catecholamines in the brains of freely moving animals. Our sensor is a 34 μm diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica. All Pinnacle CFSs require an Ag/AgCl reference electrode.
 
Our sensor is a 34 um diameter carbon fiber housed in a silica sheath that extends 0.5 mm beyond the end of the silica.

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