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当前位置: Gilson > 资料中心 > AN1013-01-基于Gilson PLC2050和VERITY®1900质谱引导分离扑热息痛和维生素C的应用(英文)

AN1013-01-基于Gilson PLC2050和VERITY®1900质谱引导分离扑热息痛和维生素C的应用(英文)

2019-07-25 11:20

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APPLICATION BENEFITS The necessity to perform purification with subsequent analysis via HPLC to determine the composition of collected fractions consumes scientists’ time and money. Utilization of multiple instruments often requires the user to learn multiple software applications. This causes scientists to spend more time learning software functionalities, and less time applying their knowledge to scientific discoveries.

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INTRODUCTION Following successful purification of small molecule drug candidates, it is necessary to pool the fractions, dry down those pooled fractions, and then prepare samples for analysis from this dried solid. Usually, the analysis of choice is a combination of analytical LCMS and NMR. To do this, it is necessary to re-dissolve the dried solid in a known quantity of a suitable solvent and then transfer small aliquots from this sample into the appropriate analysis tubes and vials.

INTRODUCTION In laboratories specializing in milk analysis, there are two methods where Gilson could help with a semi- or full automation of the complete protocols. In comparison to the manual procedure, automation provides more robust and reproducible results and sample traceability. The first method is the Copper Soap method for the calculation of the Lipolysis Index. The second method is the Amido Black method for the calculation of protein content in milk.

INTRODUCTION A general liquid handling application is to use a liquid handler as an autosampler for a spectrophotometer. The traditional manual method for filling a flow-through cuvette is to use a peristaltic pump (a sipper) to draw the sample through the cuvette. This can easily be replicated using a Gilson GX liquid handler.

INTRODUCTION Preparative LC coupled with mass spectrometry (MS) is a well-established method for purifying target compounds. The VERITY® 271 LCMS system (Figure 1) leverages the separating power of HPLC with the detection capabilities of MS to isolate fractions with high purity and achieve excellent recovery for target molecules. The system incorporates signal data from both ultraviolet (UV) and mass-based detectors.

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