通过Western Blot和免疫荧光及免疫组化的方式检测蛋白表达量

文献名： Structural, morphological and optical properties of Bi NPs obtained by laser ablation and their selective detection of L-cysteine 作者： Ramon Gabriel Teixeira Rosaa, Celso de Araujo Duartea , Wido Herwig Schreinera, Ney Pereira Mattoso Filhoa, Arandi Ginane Bezerra Jr.b, Andersson Barisonc, Fernanda Maria Marins Ocamposc a Departamento de Física, Universidade Federal do Paraná Centro Politécnico, CP 19044, 81531-990 Curitiba (PR), Brazil b Departamento Acadêmico de Física, Universidade Tecnológica Federal do Paraná, 80230-901 Curitiba (PR), Brazil c Departamento de Química, Universidade Federal do Paraná Centro Politécnico, CP 19081, 81531-990 Curitiba (PR), Brazil 摘要：In the present work we show the results of the investigation of the properties of bismuth nanoparticles (NPs) obtained by the laser ablation in water. The samples were characterized by various techniques: UV/vis spectroscopy, dynamic light scattering, transmission electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, nuclear magnetic resonance and zeta potential. The NPs were metallic and surface oxidated, exhibiting absorption peak at 257 nm due to the surface plasmon resonance. The interaction between the NPs and various amino acids was studied and revealed a selective sensibility to cysteine on a range of concentration from 80 to 720 μM, which we attribute to a dimerization of cysteine to cystine probably complexed to the nanoparticle surface by electrostatic interactions, leading to the modification of the NP absorption spectrum. 关键词：Bismuth; Nanoparticles; Laser ablation; Cysteine

矿物油组成中的共轭体系物质在紫外光区有较强的吸收，依据朗博 - 比尔定律，在256nm紫外光波长，可测定油样的吸光度来确定相应油的量。 由于紫外光度法对于油种的敏感性较强，本方法的油样需采用标准物质。

本文参考生态环境部发布的标准《HJ 1261- 2022 固定污染源废气 苯系物的测定 气袋采样/直接进样-气相色谱法》，使用磐诺气相色谱仪，进行苯系物含量测定。

双螺杆挤出技术是一种用于聚合物行业新材料开发的成熟技术。作为一种高效且连续的 混合工艺，其可以提供高灵活性并对工艺参数进行严格控制。近年来，该技术已推广到其 他应用领域，制药和食品行业加大了对挤出工艺的采用力度。在这些行业中，要完全发挥应用潜力，需要满足特定的设备要求并使用专用附件。 赛默飞可提供采用卫生级设计的专用双螺杆挤出机和附件，帮助研究人员开发下一代新型食品、生物材料和药物。