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人蔗糖酶异麦芽糖酶检测试剂盒都是一个试剂盒对应检测一个指标。不存在可以用一个试剂盒同时检测出多个指标。进口原装的有96T的,进口分装的有48T 和 96T的 ,这个得根据老师自己的需要来选择试剂盒的大小和规格,一般96T的可以测85个样本,48T的可以做37次实验,ELISA试剂盒还有11个孔是做标准曲线分析实验数据用的。试剂盒选购好了之后就可以开始测了,ELISA实验必要的一种仪器-----酶标仪!
人蔗糖酶异麦芽糖酶检测试剂盒问:
1 请问为什么有的试剂盒要求反应过夜,而有的则只需要1h?反应时间不同是由什么决定的呢?
2 稀释的抗体如何保持其稳定性?
3 显色体系(加H2O2后)的稳定性如何保持?
4 如何克服批间差异较大的缺陷?
人蔗糖酶异麦芽糖酶检测试剂盒答:
确定的反应工艺有关,一般37度1小时足够了。
稀释道抗体的话,一般都会在稀释液中增加一些无关蛋白,使稀释液有一定的蛋白浓度,也可以添加一些蛋白稳定剂,但关键还是看抗体本身的稳定性怎样。
一般目前国内的显色系统都是将TMB(OPD)和过氧化氢放开保存的。
批间差异大的问题,第一就是要原料质量过关,原料的批和批之间差异不能太大,还有就是用于生产的工艺要简单,人蔗糖酶异麦芽糖酶检测试剂盒重复性好,这样可以尽量减少批间差异。
KL-Salusinβ-Hu人Salusinβ蛋白(Salusinβ)检测试剂盒human Salusin BetaHomo sapiens human24.69-2000pg/mL8.55pg/mL96T12 months
KL-MGMT-Hu人O-6-甲基鸟嘌呤DNA甲基转移酶(MGMT)检测试剂盒human O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA KitHomo sapiens human0.156-10ng/mL0.051ng/mL96T12 months
KL-IL28B-Hu人白介素28B(IL28B)检测试剂盒human Interleukin 28B (IL28B) ELISA KitHomo sapiens human15.625-1000pg/mL5.6pg/mL96T12 months
KL-IL29-Hu人白介素29(IL29)检测试剂盒human Interleukin 29 (IL29) ELISA KitHomo sapiens human15.625-1000pg/mL6.6pg/mL96T12 months
KL-CKB-Mu小鼠脑型肌酸激酶(CKB)检测试剂盒mouse Creatine Kinase, Brain (CKB) ELISA KitMus musculus mouse15.625-1000pg/mL6.9pg/mL96T12 months
KL-IL4R-Hu人白介素4受体(IL4R)检测试剂盒human Interleukin 4 Receptor (IL4R) ELISA KitHomo sapiens human15.625-1000pg/mL5.8pg/mL96T12 months
KL-APOC4-Hu人载脂蛋白C4(APOC4)检测试剂盒human Apolipoprotein C4 (APOC4) ELISA KitHomo sapiens human7.813-500pg/mL3.7pg/mL96T12 months
KL-IGF1-Si猴胰岛素样生长因子1(IGF1)检测试剂盒Monkey Insulin Like Growth Factor 1 (IGF1) ELISA KitRhesus monkey Simian3.125-200 ng/mL0.78 ng/mL96T12 months
KL-CD14-Hu人CD14分子(CD14)检测试剂盒human Cluster Of Differentiation 14 (CD14) ELISA KitHomo sapiens human9.375-600ng/mL3.3ng/mL96T12 months
KL-ENK-Ra大鼠脑啡肽(ENK)检测试剂盒Rat Enkephalin (ENK) ELISA KitRattus norvegicus Rat74.07-6000pg/mL25.13pg/mL96T12 months
KL-aFPL3-Hu人甲胎蛋白异质体3(αFPL3)检测试剂盒human Alpha-Fetoprotein Lens Culinaris Agglutinin 3 (aFPL3) ELISA KitHomo sapiens human0.625-40ng/mL0.236ng/mL96T12 months
KL-MT1-Hu人金属硫蛋白1(MT1)检测试剂盒human Metallothionein 1 (MT1) ELISA KitHomo sapiens human15.625-1000pg/mL6.2pg/mL96T12 months
KL-Arg-Hu人精氨酸酶(Arg)检测试剂盒human Arginase (Arg) ELISA KitHomo sapiens human3.125-200ng/mL1.35ng/mL96T12 months
KL-DNASE1-Hu人脱氧核糖核酸酶Ⅰ(DNASE1)检测试剂盒human Deoxyribonuclease I (DNASE1) ELISA KitHomo sapiens human1.563-100ng/mL0.65ng/mL96T12 months
KL-CDHOB-Hu人造骨细胞钙黏蛋白(CDHOB)检测试剂盒human Cadherin, Osteoblast (CDHOB) ELISA KitHomo sapiens human0.313-20ng/mL0.117ng/mL96T12 months
KL-ADH-Hu人抗利尿激素(ADH)检测试剂盒human Antidiuretic Hormone (ADH) ELISA KitHomo sapiens human12.35-1000pg/mL4.68pg/mL96T12 months
KL-PLA1-Hu人磷脂酶A1(PLA1)检测试剂盒human Phospholipase A1 (PLA1) ELISA KitHomo sapiens human7.813-500pg/mL3.4pg/mL96T12 months
KL-FGa-Hu人纤维蛋白原α(FGα)检测试剂盒human Fibrinogen Alpha (FGa) ELISA KitHomo sapiens human0.313-20ng/mL0.145ng/mL96T12 months
KL-TBP2-Hu人硫氧还蛋白结合蛋白2(TBP2)检测试剂盒human Thioredoxin Binding Protein 2 (TBP2) ELISA KitHomo sapiens human0.156-10ng/mL0.066ng/mL96T12 months
KL-DNM2-Hu人发动蛋白2(DNM2)检测试剂盒human Dynamin 2 (DNM2) ELISA KitHomo sapiens human0.313-20ng/mL0.106ng/mL96T12 months
KL-PLSCR2-Hu人磷脂爬行酶2(PLSCR2)检测试剂盒human Phospholipid Scramblase 2 (PLSCR2) ELISA KitHomo sapiens human0.313-20 ng/mL0.09 ng/mL96T12 months
KL-VGF-Hu人VGF神经生长因子诱导蛋白(VGF)检测试剂盒human VGF Nerve Growth Factor Inducible (VGF) ELISA KitHomo sapiens human7.813-500pg/mL3.2pg/mL96T12 months
KL-ENK-Hu人脑啡肽(ENK)检测试剂盒human Enkephalin (ENK) ELISA KitHomo sapiens human98.77-8000pg/mL36.45pg/mL96T12 months
KL-LMWK-Hu人低分子量激肽原(LMWK)检测试剂盒human Low Molecular Weight Kininogen (LMWK) ELISA KitHomo sapiens human6.25-400ng/mL2.54ng/mL96T12 months
KL-CLU-Hu人簇集蛋白(CLU)检测试剂盒human Clusterin (CLU) ELISA KitHomo sapiens human0.781-50ng/mL0.37ng/mL96T12 months
KL-GLUT1-Hu人葡萄糖转运蛋白1(GLUT1)检测试剂盒human Glucose Transporter 1 (GLUT1) ELISA KitHomo sapiens human0.313-20ng/mL0.123ng/mL96T12 months
KL-NKB-Hu人神经激肽B(NKB)检测试剂盒human Neurokinin B (NKB) ELISA KitHomo sapiens human9.88-800pg/mL3.12pg/mL96T12 months
KL-NT-Hu人神经降压素(NT)检测试剂盒human Neurotensin (NT) ELISA KitHomo sapiens human3.7-300pg/mL1.35pg/mL96T12 months
KL-ADM-Hu人肾上腺髓质素(ADM)检测试剂盒human Adrenomedullin (ADM) ELISA KitHomo sapiens human12.35-1000pg/mL4.77pg/mL96T12 months
KL-MAPK14-Hu人丝裂原激活蛋白激酶14(MAPK14)检测试剂盒human Mitogen Activated Protein Kinase 14 (MAPK14) ELISA KitHomo sapiens human1.563-100ng/mL0.56ng/mL96T12 months
KL-GHITM-Hu人生长激素诱导跨膜蛋白(GHITM)检测试剂盒human Growth Hormone Inducible Transmembrane Protein (GHITM) ELISA KitHomo sapiens human0.313-20 ng/mL0.13 ng/mL96T12 months
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