急性高血糖和胰岛素干预对窦房结组织所使用的微电极阵列的自发场潜力的影响

YU FENG, HUI CAO, and YANBIN ZHANG, J. Mech. Med. Biol. DOI: 10.1142/S0219519415500463
EFFECTS OF ACUTE HYPERGLYCEMIA AND INSULIN INTERVENTION ON THE SPONTANEOUS FIELD POTENTIAL OF SINOATRIAL NODE TISSUE BY USING MICROELECTRODE ARRAYS


YU FENG
State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Xi'an Jiaotong University,P. R. China

HUI CAO
·  Corresponding author.
·  State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Xi'an Jiaotong University,P. R. China

YANBIN ZHANG
·  State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Xi'an Jiaotong University,P. R. China

Received: 18 July 2014
Revised: 8 October 2014
Published: 12 March 2015

Traditional studies on the relationship between hyperglycemia and heart diseases generally focused on the impact of chronic and long-term effect of diabetes on cardiac functions. Most of the methods were culturing myocardial cells and giving outside stimulations. However, recent studies show that acute hyperglycemia might play a significant role in spontaneous cardiac electrophysiology. In this research we applied microelectrode arrays (MEA) to record the spontaneous sinoatrial node field potentials of C57/BL6J mice and analyzed the effects of different glucose concentrations in time domain and frequency domain by using statistical method, vector maps and fast Fourier transform (FFT). Meanwhile, we studied the effects of insulin interference in the experimental process. When the concentration of the glucose solution was greater than 40 mmol/L, the spontaneous sinoatrial node field potential changed markedly. In the time domain, the amplitude decreased rapidly and the conductive characteristics were disordered. In the frequency domain, the two spectrum peaks decreased rapidly. These changes were irreversible. However, insulin preconditioning could inhibit the impact of high glucose.

高血糖与心脏疾病之间的关系的传统研究通常集中于糖尿病对心脏功能的慢性和长期效应的影响。心肌细胞培养的大多数方法，并给外界的刺激。然而，最近的研究表明，急性高血糖可能发挥重要作用，在自发的心脏电生理。在本研究中我们应用微电极阵列（MEA） 记录的 C57/BL6J 小鼠自发性窦房结场电位，并采用统计法、 矢量地图、 快速傅里叶变换 （FFT） 分析在时间域和频率域的不同糖浓度的影响。同时，我们研究了在实验过程中胰岛素干扰的影响。葡萄糖溶液的浓度大于 40 mmol/L 时，自发窦房结电位明显变化。在时域、 幅值迅速下降和导电特性被扰乱。在频率域内，两个谱峰会迅速下降。这些变化是不可逆转的。然而，胰岛素预处理能抑制高血糖的影响。
Keywords: Acute hyperglycemia; spontaneous field potential; sinoatrial node; insulin intervention; microelectrode arrays