血管内皮生长因子A(VEGFA)多克隆抗体

血管内皮生长因子A(VEGFA)多克隆抗体 

Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species    Mus musculus (Mouse)    

Product No.    yb143Mu01    

Clonality    Polyclonal    

Host    Rabbit    

Immunoglobulin Type    IgG    

Purification    Affinity Chromatography    

Applications    WB, ICC, IHC-P, IHC-F, ELISA    

Concentration    200ug/ml    

UOM    100ug    

Conjugate    No Conjugate    

IMMUNOGEN INFORMATION

Immunogen: Recombinant VEGFA (Ala27~Asn190) expressed in E.coli.

ANTIBODY SPECIFITY

The antibody is a rabbit polyclonal antibody raised against VEGFA. It has been selected for its ability to recognize VEGFA in immunohistochemical staining and western blotting.

APPLICATIONS

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

CONTENTS

Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.

IHC Images

Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue

QUALITY CONTROL

Content: The quality control contains recombinant VEGFA (Ala27~Asn190) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

STORAGE

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.