Human IL-28 ELISA kit
2015-09-18 10:06
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资料摘要:
Human IL-28
uman IL-28FOR RESEARCH USE ONLY
Assay range:6pg/ml-280pg/ml 96 determinations
Purpose
This kit allows for the determination of IL-28 concentrations in Human serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay HumanIL-28 level in the sample,use Purified Human IL-28 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-28 to wells, Combined IL-28 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human IL-28 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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人白细胞介素28 IL-28 英文说明书.doc
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