小鼠骨钙素(OC)ELISA试剂盒
2015-09-11 18:29
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资料摘要:
Mouse Osteocalcin(OC)
FOR RESEARCH USE ONLY
Assay range:3μg/L -120μg/L 96 determinations
Purpose
This kit allows for the determination of OC concentrations in Mouse serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Mouse OC level in the sample,use Purified Mouse OC antibody to coat microtiter plate wells, make solid-phase antibody, then add OC to wells, Combined OC antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse OC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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