Wako 300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药现货

【产品名称】Phos-tag (TM) Acrylamide AAL-107 2mg

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

【制造商】NARD INSTITUTE, LTD.

【销售商代码】300-93523

【规格】2mg

【价格】咨询订购400-8II-9081

【保存条件】Keep at RT.

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

【注意事项】This product is for research use only. Do not administer it to human.

【摘要】This product is for research use only. Do not administer it to human.

Wako has taken over the distribution of Phos-tag products from those manufacturers NARD Institute, Ltd. and Manac Inc. in May, 2010.

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

Phos-tag technology

Phos-tag is a novel phosphate-binding tag at neutral pH (physiological pH) and was developed by Department of Functional Molecular Science at Hiroshima University. Phos-tag technologies are innovative methods using Phos-tag for separation, purification, and detection of phosphorylated substances (protein, peptide, etc.).

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

Under neutral pH conditions (physiological conditions), Phos-tag binds with an anionic substituent, especially strongly with a phosphomonoester dianion. Phos-tag can take the place of conventional enzyme immunoassay and radioactive isotope methods as an agent for capturing substances with an anionic substituent, especially a phosphomonoester dianion. Unstable phosphorylated compounds, which were difficult to measure up until now due to instability, can also be stabilized.

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

The phosphorylation and dephosphorylation of proteins are important themes relevant to information transmission in living bodies and are crucial for life functions. The clarification of such phosphorylation processes is a major theme of post-genome research and is targeted in the development of new drugs.

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

Phos-tag technology makes use of the properties of Phos-tag, that is, the properties of binding strongly under neutral pH conditions (physiological conditions) to anionic substituents, especially phosphomonoester dianions, and takes the place of conventional enzyme immunoassay and radioactive isotope methods as a technology for capturing substances with an anionic substituent, especially a phosphomonoester dianion, as well as stabilizing unstable phosphorylated compounds, which were difficult to measure up until now due to instability. Generally with a captured compound, the captured anionic compound dissociates at a pH of 3 to 4.

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

Applications

The properties of Phos-tag, which enable labeling of most compounds having a phosphate group in the molecule, such as proteins, nucleic acids, and other biochemical substances, can be put to use in the following applications.

Application (1): Electrophoresis:

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

Phos-tag Acrylamide (AAL-107) [Wako Cat. #304-93521 (10 mg)]? Protocol

Phos-tag Acrylamide (AAL-107) [Wako Cat. #300-93523 (2 mg)]? Protocol

Application (2): Surface plasmon resonance (SPR):

Phos-tag Biotin BTL-104 [Wako Cat. #301-93531 (10 mg)]? Protocol

Phos-tag Biotin BTL-105 [Wako Cat. #304-93541 (10 mg)]? Protocol

Application (3): Western Blotting:

Phos-tag Biotin BTL-104 [Wako Cat. #301-93531 (10 mg)]? Protocol

Phos-tag Biotin BTL-105 [Wako Cat. #304-93541 (10 mg)]? Protocol

Application (4): Mass spectrometry (MS):

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

Phos-tag Mass Analytical Kit (MS-101KIT) [Wako Cat. #305-93551 (1 set)]?Protocol

Application (5): Enrichment, Separation and Purification:

Phos-tag Agarose AG-501 [Wako Cat. #302-93561 (0.5 mL)]? Protocol

Phos-tag Agarose AG-503 [Wako Cat. #308-93563 (3 mL)]? Protocol

Application (6): NMR

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

Application (7): Deactivators

Application (8): Enzyme inhibitors

【参考文献】

"Recognition of phosphate monoester dianion by an alkoxide-bridged dinuclear zinc (II) complex", E. Kinoshita, M. Takahashi, H. Takeda, M. Shiro, and T. Koike, Dalton Trans., 21, 1189-1193 (2004).

"Phosphate-binding tag, a new tool to visualize phosphorylated proteins", E. Kinoshita, E. Kinoshita-Kikuta, K. Takiyama, and T. Koike, Mol. Cell.

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

Proteomics, 5, 749-757 (2006).

"A single nucleotide polymorphism genotyping method using phosphate-affinity polyacrylamide gel electrophoresis", E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike, Anal. Biochem., 361, 294-298 (2007).

"Label-free kinase profiling using phosphate affinity polyacrylamide gel electrophoresis", E. Kinoshita-Kikuta, Y. Aoki, E. Kinoshita, and T. Koike, Mol. Cell. Proteomics, 6, 356-366 (2007).

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

"Separation of a phosphorylated-His protein using phosphate-affinity Polyacrylamide gel", S. Yamada, H. Nalkamura, E. Kinoshita, E. Kinoshita-Kikuta, T. Koike, and Y. Shiro, Anal. Biochem., 360, 160-162 (2007).

"A mobility-shift detection method for DNA methylation analysis using phosphate-affinity polyacrylamide gel electrophoresis", E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike, Anal. Biochem., 378, 102-104 (2008).

"Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE", E. Kinoshita, E. Kinoshita-Kikuta, M. Matsubara, S. Yamada, H. Nakamura, Y. Shiro, Y. Aoki, K. Okita, and T. Koike, Proteomics, 8, 2994-3003 (2008).

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

"FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway", M. Ishiai, H. Kitao, A. Smogorzewska, J. Tomida, A. Kinomura, E. Uchida, A. Saberi, E. Kinoshita, E. Kinoshita-Kikuta, T. Koike, S. Tashiro, S. J. Elledge, and M. Takata, Nature Struct. Mol. Biol., 15, 1138-1146 (2008).

"Two-dimensional phosphate affinity gel electrophoresis for the analysis of phosphoprotein isotypes", E. Kinoshita, E. Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

Kinoshita-Kikuta, M. Matsubara, Y. Aoki, S. Ohie, Y. Mouri, and T. Koike, Electrophoresis, 30, 550-559 (2009).

"Phosphate-affinity Gel Electrophoresis Using a Phos-tag Molecule for Phosphoproteome Study", E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike, Current Proteomics, 6, 104-121 (2009).

"Mobility shift detection of phosphorylation on large proteins using a Phos-tag SDS-PAGE gel strengthened with agarose", E. Kinoshita, E. Kinoshita-Kikuta, H. Ujihara, and T. Koike, Proteomics, 9, 4098-101 (2009).

"Separation and detection of large phosphoproteins by using Phos-tag SDS-PAGE", E. Kinoshita, E. Kinoshita-Kikuta, Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货'

and T. Koike, Nature Protocols, 4, 1513-21 (2009).

上海子起_生物常年提供以下产品 现货或准现货！订购热线：4OO-811-9081

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298-65701   LabAssay? Glucose   1000tests     -

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

300-93523   Phos-tag (TM) Acrylamide AAL-107      2mg      -

301-93531   Phos-tag (TM) Biotin BTL-104 10mg    -

301-99413   Phos-tagTM Biotin BTL-104 1mM Aqueous Solution[for Campaign]     0.1mL      753451-66-0

Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货

302-93522   Phos-tagTM Acrylamide AAL-107 5mM Aqueous Solution[for Campaign]      0.3mL    871839-54-2

631-00651   Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)     20mL    -

637-00653   Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)     100mL   -

637-00653   Cellmatrix Type I -A (Collagen, Type I, 3 mg/mL, pH 3.0)     100mL   -

638-01021   Endotoxin Standad /JPSE  10000un      -

986-10001   Anti asialo GM1 (Rabbit), (014-09801)  1mL      617-45-8

 Wako  300-93523 Phos-tag (TM) Acrylamide AAL-107 2mg 和光纯药 现货