Wako 125-02543 Lysyl Endopeptidase? 现货

Wako  125-02543 Lysyl Endopeptidase? 现货

【产品名称】,

Wako  125-02543 Lysyl Endopeptidase? 现货

【制造商】Wako Pure Chemical Industries, Ltd.

【销售商代码】125-02543

【规格】1vial(2AU)

【价格】咨询 4OO-811-9081

【分子量】27,500

【保存条件】Keep at RT.

【外观】

Wako  125-02543 Lysyl Endopeptidase? 现货

【注意事项】This product is for research use only. Do not administer it to human.

Lysyl Endopeptidase, originally isolated from the soil bacterium discovered by Masaki, et al. cleaves specifically the peptide bonds at the carboxy-terminal side of Lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for protein sequence analysis and for enzymatic synthesis of Lys-X compounds. An added feature of Lysyl Endopeptidase is its ability to retain complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 degrees C.

Unit definition: One amidase unit (AU) is the amount of enzyme, which will produce 1 micromole of p-Nitroaniline per minute at 30 degrees C, pH 9.5.

Wako  125-02543 Lysyl Endopeptidase? 现货

【Source】 Bacteria

Originally, the source of this product was indicated as "Achromobacter lyticus" based on the physiological and morphological properties of the bacteria. However, we confirmed that the 16SrDNA sequence was highly homologous to that of Lysobacter.

【Appearance】Lyophilized form containing ca. 10% Tris-HCl buffer, pH 8.

【Activity】 Shown on each label

【Molecular Weight:】27,000 (gel filtration); 30,000 (SDS electrophoresis)

【Solubility:】Soluble in water or buffer solution.

 Wako  125-02543 Lysyl Endopeptidase? 现货

Stability: Stable at 4 degrees C, when dissolved in buffer of pH 5~12. Stable at 30 degrees C in the range of pH 6~11, but unstable at 50 degrees C or higher.

Optimal pH: 9.0 -9.5 (Amidase activity)

Isoelectric point: 6.9~7.0

Substrate specificity:

Wako  125-02543 Lysyl Endopeptidase? 现货

Hydrolysable substrate ... Tos-Lys-Ome, Bz-Lys-NH2, Bz-Lys-pNA, Lys-pNA

Unhydrolysable substrate ... Bz-Arg-NH2, Bz-Arg-pNa, Arg-pNA

【Inhibitors】 DFP, PMSF, TLCK

【用途】for Biochemistry

 Wako  125-02543 Lysyl Endopeptidase? 现货

上海子起_生物常年提供以下产品 现货或准现货！订购热线：4OO-811-9081

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