金属硫蛋白3抗体
『别名』Mt3; Mt-3; GIFB; GRIF; Metallothionein III; GIF; Growth inhibitory factor; Metallothionein 3; Metallothionein3; Mt 3; MT III; Metallothionein-3; Growth inhibitory factor; Metallothionein-III; MT-III.
『浓度』金属硫蛋白3抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
金属硫蛋白3抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
金属硫蛋白3抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAE002Ra01 CREB结合蛋白(CREBBP)多克隆抗体 Polyclonal Antibody to CREB Binding Protein (CREBBP)
PAE002Mu01 CREB结合蛋白(CREBBP)多克隆抗体 Polyclonal Antibody to CREB Binding Protein (CREBBP)
PAE398Hu01 肌酸转运蛋白(CRT)多克隆抗体 Polyclonal Antibody to Creatine Transporter (CRT)
PAA109Bo01 肌肌酸激酶(CKM)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
PAA109Ca01 肌肌酸激酶(CKM)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
PAA109Fi01 肌肌酸激酶(CKM)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
PAA109Ga01 肌肌酸激酶(CKM)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
PAA109Hu01 肌肌酸激酶(CKM)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
PAA109Mu01 肌肌酸激酶(CKM)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
PAA109Po01 肌肌酸激酶(CKM)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
PAA109Ra01 肌肌酸激酶(CKM)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
PAA109Rb51 肌肌酸激酶(CKM)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
PAA109Mu02 肌肌酸激酶(CKM)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
PAC386Hu01 线粒体肌酸激酶2(CKMT2)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2)
PAA263Bo01 线粒体肌酸激酶1A(CKMT1A)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Mitochondrial 1A (CKMT1A)
PAA263Ga01 线粒体肌酸激酶1A(CKMT1A)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Mitochondrial 1A (CKMT1A)
PAA263Hu01 线粒体肌酸激酶1A(CKMT1A)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Mitochondrial 1A (CKMT1A)
PAA263Mu01 线粒体肌酸激酶1A(CKMT1A)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Mitochondrial 1A (CKMT1A)
PAA263Po01 线粒体肌酸激酶1A(CKMT1A)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Mitochondrial 1A (CKMT1A)
PAC030Hu01 脑型肌酸激酶(CKB)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Brain (CKB)
PAC030Mu01 脑型肌酸激酶(CKB)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Brain (CKB)
PAC030Ca01 脑型肌酸激酶(CKB)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Brain (CKB)
PAC030Ra01 脑型肌酸激酶(CKB)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Brain (CKB)
PAC030Mu02 脑型肌酸激酶(CKB)多克隆抗体 Polyclonal Antibody to Creatine Kinase, Brain (CKB)
PAC232Hu01 C-Raf原癌基因丝苏氨酸蛋白激酶(CRAF)多克隆抗体 Polyclonal Antibody to C-Raf Proto Oncogene Serine/Threonine Protein Kinase (CRAF)
PAA447Hu01 C-肽(CP)多克隆抗体 Polyclonal Antibody to C-Peptide (CP)
PAA447Ga01 C-肽(CP)多克隆抗体 Polyclonal Antibody to C-Peptide (CP)
PAA447Po01 C-肽(CP)多克隆抗体 Polyclonal Antibody to C-Peptide (CP)
PAJ305Hu01 柯萨奇病毒腺病毒受体(CXADR)多克隆抗体 Polyclonal Antibody to Coxsackie Virus And Adenovirus Receptor (CXADR)
PAW786Hu01 柯萨奇病毒腺病毒受体样膜蛋白(CLMP)多克隆抗体 Polyclonal Antibody to Coxsackie And Adenovirus Receptor Like Membrane Protein (CLMP)
PAQ185Hu01 耦合因子6(CF6)多克隆抗体 Polyclonal Antibody to Coupling Factor 6 (CF6)
PAQ185Ra01 耦合因子6(CF6)多克隆抗体 Polyclonal Antibody to Coupling Factor 6 (CF6)
PAC393Hu01 皮质醇稳定蛋白(CORT)多克隆抗体 Polyclonal Antibody to Cortistatin (CORT)
PAA067Ge01 可的松(COR)多克隆抗体 Polyclonal Antibody to Cortisone (COR)
PAA462Ge01 皮质醇(Cor)多克隆抗体 Polyclonal Antibody to Cortisol (Cor)
PAD513Ra01 促肾上腺皮质素释放激素受体2(CRHR2)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone Receptor 2 (CRHR2)
PAD513Hu01 促肾上腺皮质素释放激素受体2(CRHR2)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone Receptor 2 (CRHR2)
PAC935Ra01 促肾上腺皮质素释放激素受体1(CRHR1)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone Receptor 1 (CRHR1)
PAC935Hu01 促肾上腺皮质素释放激素受体1(CRHR1)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone Receptor 1 (CRHR1)
PAC401Hu01 促肾上腺皮质素释放激素结合蛋白(CRHBP)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone Binding Protein (CRHBP)
PAC401Ra01 促肾上腺皮质素释放激素结合蛋白(CRHBP)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone Binding Protein (CRHBP)
PAA835Hu01 促肾上皮质激素释放激素(CRH)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)
PAA835Mu01 促肾上皮质激素释放激素(CRH)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)
PAA835Ra01 促肾上皮质激素释放激素(CRH)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)
PAA835Mu02 促肾上皮质激素释放激素(CRH)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)
PAA835Ra02 促肾上皮质激素释放激素(CRH)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)
PAA835Hu02 促肾上皮质激素释放激素(CRH)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)
PAA835Hu03 促肾上皮质激素释放激素(CRH)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)
PAA835Hu04 促肾上皮质激素释放激素(CRH)多克隆抗体 Polyclonal Antibody to Corticotropin Releasing Hormone (CRH)
PAA540Ge01 皮质酮(Cort)多克隆抗体 Polyclonal Antibody to Corticosterone (Cort)
PAB226Hu01 皮质醇结合球蛋白(CBG)多克隆抗体 Polyclonal Antibody to Corticosteroid Binding Globulin (CBG)
PAB226Mu01 皮质醇结合球蛋白金属硫蛋白3抗体(CBG)多克隆抗体 Polyclonal Antibody to Corticosteroid Binding Globulin (CBG)
PAB226Ra01 皮质醇结合球蛋白(CBG)多克隆抗体 Polyclonal Antibody to Corticosteroid Binding Globulin (CBG)
PAB226Hu02 皮质醇结合球蛋白(CBG)多克隆抗体 Polyclonal Antibody to Corticosteroid Binding Globulin (CBG)
PAB226Ra02 皮质醇结合球蛋白(CBG)多克隆抗体 Polyclonal Antibody to Corticosteroid Binding Globulin (CBG)