复合型表皮生长因子样结构域蛋白5抗体

复合型表皮生长因子样结构域蛋白5抗体 

『别名』MEGF 5; MEGF5; Multiple EGF-like domains protein 5; Multiple epidermal growth factor like domains 5; Multiple epidermal growth factor-like domains 5; Multiple epidermal growth factor-like domains protein 5; SLIL 2; SLIL2; SLIT 1; Slit 2; Slit 3; Slit homolog 3 (Drosophila); Slit homolog 3; Slit homolog 3 protein; Slit-3 ; SLIT1; Slit2; Slit3; SLIT3_HUMAN; FLJ10764; KIAA0814. 

『浓度』复合型表皮生长因子样结构域蛋白5抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

复合型表皮生长因子样结构域蛋白5抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

复合型表皮生长因子样结构域蛋白5抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAA295Mu01 谷胱甘肽过氧化酶1(GPX1)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1)

PAA295Po01 谷胱甘肽过氧化酶1(GPX1)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1)

PAA295Ra01 谷胱甘肽过氧化酶1(GPX1)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1)

PAA295Rb51 谷胱甘肽过氧化酶1(GPX1)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1)

PAA295Mu02 谷胱甘肽过氧化酶1(GPX1)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1)

PAA294Ge01 谷胱甘肽(GSH)多克隆抗体 Polyclonal Antibody to Glutathione (GSH)

PAG979Hu01 谷氧还蛋白3(GLRX3)多克隆抗体 Polyclonal Antibody to Glutaredoxin 3 (GLRX3)

PAG979Hu02 谷氧还蛋白3(GLRX3)多克隆抗体 Polyclonal Antibody to Glutaredoxin 3 (GLRX3)

PAG979Mu01 谷氧还蛋白3(GLRX3)多克隆抗体 Polyclonal Antibody to Glutaredoxin 3 (GLRX3)

PAG981Hu01 谷氧还蛋白(GLRX)多克隆抗体 Polyclonal Antibody to Glutaredoxin (GLRX)

PAG981Mu01 谷氧还蛋白(GLRX)多克隆抗体 Polyclonal Antibody to Glutaredoxin (GLRX)

PAG981Mu02 谷氧还蛋白(GLRX)多克隆抗体 Polyclonal Antibody to Glutaredoxin (GLRX)

PAA854Ra01 谷氨氨肽酶(GluAP)多克隆抗体 Polyclonal Antibody to Glutamyl Aminopeptidase (GluAP)

PAA854Hu01 谷氨氨肽酶(GluAP)多克隆抗体 Polyclonal Antibody to Glutamyl Aminopeptidase (GluAP)

PAA854Mu01 谷氨氨肽酶(GluAP)多克隆抗体 Polyclonal Antibody to Glutamyl Aminopeptidase (GluAP)

PAH274Hu01 谷氨酰胺酰肽环转移酶(QPCT)多克隆抗体 Polyclonal Antibody to Glutaminyl Peptide Cyclotransferase (QPCT)

PAD761Hu01 谷氨酰胺合成酶(GS)多克隆抗体 Polyclonal Antibody to Glutamine synthetase (GS)

PAD761Mu01 谷氨酰胺合成酶(GS)多克隆抗体 Polyclonal Antibody to Glutamine synthetase (GS)

PAD761Ra01 谷氨酰胺合成酶(GS)多克隆抗体 Polyclonal Antibody to Glutamine synthetase (GS)

PAD761Mu02 谷氨酰胺合成酶(GS)多克隆抗体 Polyclonal Antibody to Glutamine synthetase (GS)

PAD864Hu01 谷氨酰胺果糖-6-磷酸转氨酶2(GFPT2)多克隆抗体 Polyclonal Antibody to Glutamine Fructose-6-Phosphate Transaminase 2 (GFPT2)

PAJ025Mu01 谷氨酰胺酶2(GLS2)多克隆抗体 Polyclonal Antibody to Glutaminase 2 (GLS2)

PAJ025Mu02 谷氨酰胺酶2(GLS2)多克隆抗体 Polyclonal Antibody to Glutaminase 2 (GLS2)

PAJ026Hu01 谷氨酰胺酶(GLS)多克隆抗体 Polyclonal Antibody to Glutaminase (GLS)

PAJ026Mu01 谷氨酰胺酶(GLS)多克隆抗体 Polyclonal Antibody to Glutaminase (GLS)

PAJ026Mu02 谷氨酰胺酶(GLS)多克隆抗体 Polyclonal Antibody to Glutaminase (GLS)

PAJ026Ra01 谷氨酰胺酶(GLS)多克隆抗体 Polyclonal Antibody to Glutaminase (GLS)

PAS122Ge01 谷氨酸(Glu)多克隆抗体 Polyclonal Antibody to Glutamic Acid (Glu)

PAB891Hu01 代谢型型谷氨酸受体3(GRM3)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Metabotropic 3 (GRM3)

PAE821Hu01 代谢型型谷氨酸受体1(GRM1)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Metabotropic 1 (GRM1)

PAE809Hu01 N-甲基-D-天氡氨酸离子能谷氨酸受体2D(GRIN2D)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2D (GRIN2D)

PAE808Hu01 N-甲基-D-天氡氨酸离子能谷氨酸受体2C(GRIN2C)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2C (GRIN2C)

PAE807Hu01 N-甲基-D-天氡氨酸离子能谷氨酸受体2B(GRIN2B)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B)

PAE806Hu01 N-甲基-D-天氡氨酸离子能谷氨酸受体2A(GRIN2A)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A)

PAE806Mu01 N-甲基-D-天氡氨酸离子能谷氨酸受体2A(GRIN2A)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A)

PAE806Mu02 N-甲基-D-天氡氨酸离子能谷氨酸受体2A(GRIN2A)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A)

PAE805Ra01 N-甲基-D-天氡氨酸离子能谷氨酸受体1(GRIN1)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1)

PAE804Hu01 AMPA离子能谷氨酸受体4(GRIA4)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4)

PAE804Mu01 AMPA离子能谷氨酸受体4(GRIA4)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4)

PAE804Mu02 AMPA离子能谷氨酸受体4(GRIA4)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4)

PAE803Hu01 AMPA离子能谷氨酸受体3(GRIA3)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3)

PAE803Mu01 AMPA离子能谷氨酸受体3(GRIA3)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3)

PAE803Mu02 AMPA离子能谷氨酸受体3(GRIA3)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3)

PAE802Hu01 AMPA离子能谷氨酸受体2(GRIA2)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2)

PAE802Mu01 AMPA离子能谷氨酸受体2(GRIA2)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2)

PAE802Mu02 AMPA离子能谷氨酸受体2(GRIA2)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, AMPA 2 (GRIA2)

PAE801Ra01 AMPA离子能谷氨酸受体1(GRIA1)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1)

PAE801Hu01 AMPA离子能谷氨酸受体1(GRIA1)多克隆抗体 Polyclonal Antibody to Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1)

PAB753Hu01 谷氨酸受体相互作用蛋白1(GRIP1)多克隆抗体 Polyclonal Antibody to Glutamate Receptor Interacting Protein 1 (GRIP1)

PAB753Hu02 谷氨酸受体相互作用蛋白1(GRIP1)多克隆抗体 Polyclonal Antibody to Glutamate Receptor复合型表皮生长因子样结构域蛋白5抗体 Interacting Protein 1 (GRIP1)

PAA293Bo01 谷氨酸脱氢酶(GDH)多克隆抗体 Polyclonal Antibody to Glutamate Dehydrogenase 1 (GDH)

PAA293Hu01 谷氨酸脱氢酶(GDH)多克隆抗体 Polyclonal Antibody to Glutamate Dehydrogenase 1 (GDH)

PAA293Mu01 谷氨酸脱氢酶(GDH)多克隆抗体 Polyclonal Antibody to Glutamate Dehydrogenase 1 (GDH)

PAA293Ra01 谷氨酸脱氢酶(GDH)多克隆抗体 Polyclonal Antibody to Glutamate Dehydrogenase 1 (GDH)