多药耐药相关蛋白8/9/14抗体
『别名』MRP 14; MRP-14; MRP 8; MRP8; MRP-8; NIF; P14; P8; Protein S100 A8; Protein S100 A9; S100 A8; S100 A9; S100 calcium binding protein A8; S100 calcium binding protein A9; S100A8; S100A9; Urinary stone protein band A; 60B8AG; CAGA; CAGB; Calgranulin A; Calgranulin B; Calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; CGLA; CGLB; CP 10; CP10; Cystic fibrosis antigen; L1AG; Leukocyte L1 complex heavy chain; Leukocyte L1 complex light chain; LIAG; MA387; MAC387; MIF; MRP8 + MRP14; Migration inhibitory factor related protein 14; Migration inhibitory factor related protein 8.
『浓度』多药耐药相关蛋白8/9/14抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
多药耐药相关蛋白8/9/14抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
多药耐药相关蛋白8/9/14抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAD757Ra01 谷光甘肽合成酶(GSS)多克隆抗体 Polyclonal Antibody to Glutathione Synthetase (GSS)
PAD757Mu01 谷光甘肽合成酶(GSS)多克隆抗体 Polyclonal Antibody to Glutathione Synthetase (GSS)
PAD757Mu02 谷光甘肽合成酶(GSS)多克隆抗体 Polyclonal Antibody to Glutathione Synthetase (GSS)
PAA613Hu01 谷胱甘肽S转移酶θ2(GSTθ2)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Theta 2 (GSTt2)
PAA613Mu01 谷胱甘肽S转移酶θ2(GSTθ2)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Theta 2 (GSTt2)
PAA613Ra01 谷胱甘肽S转移酶θ2(GSTθ2)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Theta 2 (GSTt2)
PAA622Hu01 谷胱甘肽S转移酶θ1(GSTθ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Theta 1 (GSTt1)
PAA622Mu01 谷胱甘肽S转移酶θ1(GSTθ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Theta 1 (GSTt1)
PAA622Ra01 谷胱甘肽S转移酶θ1(GSTθ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Theta 1 (GSTt1)
PAB090Hu01 谷胱甘肽S转移酶π1(GSTπ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Pi (GSTp)
PAB090Po01 谷胱甘肽S转移酶π1(GSTπ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Pi (GSTp)
PAB090Bo01 谷胱甘肽S转移酶π1(GSTπ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Pi (GSTp)
PAB090Mu01 谷胱甘肽S转移酶π1(GSTπ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Pi (GSTp)
PAB090Ra01 谷胱甘肽S转移酶π1(GSTπ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Pi (GSTp)
PAA623Hu01 谷胱甘肽S转移酶ω1(GSTω1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Omega 1 (GSTo1)
PAA623Mu01 谷胱甘肽S转移酶ω1(GSTω1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Omega 1 (GSTo1)
PAA623Mu02 谷胱甘肽S转移酶ω1(GSTω1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Omega 1 (GSTo1)
PAA643Hu01 谷胱甘肽S转移酶μ4(GSTμ4)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Mu 4 (GSTm4)
PAA654Hu01 谷胱甘肽S转移酶μ3(GSTμ3)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Mu 3, Brain (GSTm3)
PAA657Hu01 谷胱甘肽S转移酶μ2(GSTμ2)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Mu 2, Muscle (GSTm2)
PAA657Ra01 谷胱甘肽S转移酶μ2(GSTμ2)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Mu 2, Muscle (GSTm2)
PAA657Mu01 谷胱甘肽S转移酶μ2(GSTμ2)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Mu 2, Muscle (GSTm2)
PAA658Ga01 谷胱甘肽S转移酶μ1(GSTμ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Mu 1 (GSTm1)
PAA658Mu01 谷胱甘肽S转移酶μ1(GSTμ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Mu 1 (GSTm1)
PAA658Ra01 谷胱甘肽S转移酶μ1(GSTμ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Mu 1 (GSTm1)
PAA658Hu01 谷胱甘肽S转移酶μ1(GSTμ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Mu 1 (GSTm1)
PAB089Hu01 谷胱甘肽S转移酶κ1(GSTκ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Kappa 1 (GSTk1)
PAB089Mu01 谷胱甘肽S转移酶κ1(GSTκ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Kappa 1 (GSTk1)
PAB089Ra01 谷胱甘肽S转移酶κ1(GSTκ1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Kappa 1 (GSTk1)
PAB956Hu01 谷胱甘肽S转移酶α5(GSTα5)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 5 (GSTa5)
PAB956Ra01 谷胱甘肽S转移酶α5(GSTα5)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 5 (GSTa5)
PAA659Hu01 谷胱甘肽S转移酶α4(GSTα4)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 4 (GSTa4)
PAA659Mu01 谷胱甘肽S转移酶α4(GSTα4)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 4 (GSTa4)
PAA659Mu02 谷胱甘肽S转移酶α4(GSTα4)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 4 (GSTa4)
PAA659Ra01 谷胱甘肽S转移酶α4(GSTα4)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 4 (GSTa4)
PAA671Ra01 谷胱甘肽S转移酶α3(GSTα3)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 3 (GSTa3)
PAA671Hu01 谷胱甘肽S转移酶α3(GSTα3)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 3 (GSTa3)
PAA671Mu01 谷胱甘肽S转移酶α3(GSTα3)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 3 (GSTa3)
PAB957Hu01 谷胱甘肽S转移酶α2(GSTα2)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 2 (GSTa2)
PAB957Mu01 谷胱甘肽S转移酶α2(GSTα2)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 2 (GSTa2)
PAA609Hu01 谷胱甘肽S转移酶α1(GSTα1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 1 (GSTa1)
PAA609Mu01 谷胱甘肽S转移酶α1(GSTα1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 1 (GSTa1)
PAA609Ra01 谷胱甘肽S转移酶α1(GSTα1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 1 (GSTa1)
PAA609Mu02 谷胱甘肽S转移酶α1(GSTα1)多克隆抗体 Polyclonal Antibody to Glutathione S Transferase Alpha 1 (GSTa1)
PAB314Hu01 谷胱甘肽还原酶(GR)多克隆抗体 Polyclonal Antibody to Glutathione Reductase (GR)
PAB314Ra01 谷胱甘肽还原酶(GR)多克隆抗体 Polyclonal Antibody to Glutathione Reductase (GR)
PAB314Mu01 谷胱甘肽还原酶(GR)多克隆抗体 Polyclonal Antibody to Glutathione Reductase (GR)
PAB314Mu02 谷胱甘肽还原酶(GR)多克隆抗体 Polyclonal Antibody to Glutathione Reductase (GR)
PAD994Ra01 谷胱甘肽过氧化酶5(GPX5)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 5 (GPX5)
PAC994Hu01 谷胱甘肽过氧化酶4(GPX4)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 4 (GPX4)
PAC994Mu01 谷胱甘肽过氧化酶4(GPX4)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 4 (GPX4)
PAC992Hu01 血浆谷胱甘肽过氧化物酶(GPX3)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3)
PAC992Ra01 多药耐药相关蛋白8/9/14抗体血浆谷胱甘肽过氧化物酶(GPX3)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3)
PAC992Mu01 血浆谷胱甘肽过氧化物酶(GPX3)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3)
PAC993Hu01 谷胱甘肽过氧化酶2(GPX2)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 2, Gastrointestinal (GPX2)
PAA295Bo01 谷胱甘肽过氧化酶1(GPX1)多克隆抗体 Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1)