有丝分裂阻滞缺陷2样蛋白2抗体

?有丝分裂阻滞缺陷2样蛋白2抗体 

『别名』Homolog of REV7 S cerevisiae; HREV7; MAD2 homolog; MAD2 like 2; MAD2 mitotic arrest deficient like 2; MAD2B; Mitotic Arrest Deficient 2 L2; Mitotic arrest deficient homolog like 2; Mitotic arrest deficient like 2 (yeast); Mitotic arrest deficient yeast homolog; Mitotic spindle assembly checkpoint protein MAD2B; REV 7; REV7; Weakly similar to Mitotic MAD2 protein (S cerevisiae). 

『浓度』有丝分裂阻滞缺陷2样蛋白2抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

有丝分裂阻滞缺陷2样蛋白2抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

有丝分裂阻滞缺陷2样蛋白2抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAD506Ra01 70kDa热休克蛋白结合蛋白1(HSPBP1)多克隆抗体 Polyclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1)

PAA822Ga01 60kD热休克蛋白(HSPD1)多克隆抗体 Polyclonal Antibody to Heat Shock 60kD Protein 1, Chaperonin (HSPD1)

PAA822Hu01 60kD热休克蛋白(HSPD1)多克隆抗体 Polyclonal Antibody to Heat Shock 60kD Protein 1, Chaperonin (HSPD1)

PAA822Mu01 60kD热休克蛋白(HSPD1)多克隆抗体 Polyclonal Antibody to Heat Shock 60kD Protein 1, Chaperonin (HSPD1)

PAA822Ra01 60kD热休克蛋白(HSPD1)多克隆抗体 Polyclonal Antibody to Heat Shock 60kD Protein 1, Chaperonin (HSPD1)

PAA822Hu02 60kD热休克蛋白(HSPD1)多克隆抗体 Polyclonal Antibody to Heat Shock 60kD Protein 1, Chaperonin (HSPD1)

PAA822Mu02 60kD热休克蛋白(HSPD1)多克隆抗体 Polyclonal Antibody to Heat Shock 60kD Protein 1, Chaperonin (HSPD1)

PAG165Hu01 40kDa热休克蛋白2(HSPF2)多克隆抗体 Polyclonal Antibody to Heat Shock 40kDa Protein 2 (HSPF2)

PAA693Bo01 27kDa热休克蛋白(HSPB1)多克隆抗体 Polyclonal Antibody to Heat Shock 27kDa Protein 1 (HSPB1)

PAA693Hu01 27kDa热休克蛋白(HSPB1)多克隆抗体 Polyclonal Antibody to Heat Shock 27kDa Protein 1 (HSPB1)

PAA693Mu01 27kDa热休克蛋白(HSPB1)多克隆抗体 Polyclonal Antibody to Heat Shock 27kDa Protein 1 (HSPB1)

PAA693Ra01 27kDa热休克蛋白(HSPB1)多克隆抗体 Polyclonal Antibody to Heat Shock 27kDa Protein 1 (HSPB1)

PAA693Mu02 27kDa热休克蛋白(HSPB1)多克隆抗体 Polyclonal Antibody to Heat Shock 27kDa Protein 1 (HSPB1)

PAB501Hu01 10kDa热休克蛋白1(HSP10)多克隆抗体 Polyclonal Antibody to Heat Shock 10kDa Protein 1 (HSP10)

PAB501Mu01 10kDa热休克蛋白1(HSP10)多克隆抗体 Polyclonal Antibody to Heat Shock 10kDa Protein 1 (HSP10)

PAB501Mu02 10kDa热休克蛋白1(HSP10)多克隆抗体 Polyclonal Antibody to Heat Shock 10kDa Protein 1 (HSP10)

PAD511Hu01 105kDa/110kDa热休克蛋白1(HSPH1)多克隆抗体 Polyclonal Antibody to Heat Shock 105kDa/110kDa Protein 1 (HSPH1)

PAD511Ra01 105kDa/110kDa热休克蛋白1(HSPH1)多克隆抗体 Polyclonal Antibody to Heat Shock 105kDa/110kDa Protein 1 (HSPH1)

PAC519Hu01 心脏神经嵴衍生物表达蛋白1(HAND1)多克隆抗体 Polyclonal Antibody to Heart And Neural Crest Derivatives Expressed Protein 1 (HAND1)

PAM623Mu01 HAUS Augmin样复合体亚基7(HAUS7)多克隆抗体 Polyclonal Antibody to HAUS Augmin Like Complex Subunit 7 (HAUS7)

PAM623Hu01 HAUS Augmin样复合体亚基7(HAUS7)多克隆抗体 Polyclonal Antibody to HAUS Augmin Like Complex Subunit 7 (HAUS7)

PAM623Ra01 HAUS Augmin样复合体亚基7(HAUS7)多克隆抗体 Polyclonal Antibody to HAUS Augmin Like Complex Subunit 7 (HAUS7)

PAC000Hu01 触珠蛋白相关蛋白(HPR)多克隆抗体 Polyclonal Antibody to Haptoglobin Related Protein (HPR)

PAC000Hu02 触珠蛋白相关蛋白(HPR)多克隆抗体 Polyclonal Antibody to Haptoglobin Related Protein (HPR)

PAC000Mu01 触珠蛋白相关蛋白(HPR)多克隆抗体 Polyclonal Antibody to Haptoglobin Related Protein (HPR)

PAC000Ra01 触珠蛋白相关蛋白(HPR)多克隆抗体 Polyclonal Antibody to Haptoglobin Related Protein (HPR)

PAC000Hu03 触珠蛋白相关蛋白(HPR)多克隆抗体 Polyclonal Antibody to Haptoglobin Related Protein (HPR)

PAC000Ra02 触珠蛋白相关蛋白(HPR)多克隆抗体 Polyclonal Antibody to Haptoglobin Related Protein (HPR)

PAA817Hu01 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAA817Mu01 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAA817Ra01 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAA817Gu01 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAA817Rb53 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAA817Bo01 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAA817Po01 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAA817Rb51 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAA817Ca01 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAA817Hu02 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAA817Rb52 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAA817Mu02 结合珠蛋白(Hpt)多克隆抗体 Polyclonal Antibody to Haptoglobin (Hpt)

PAK289Hu01 Split多毛增强子1(HES1)多克隆抗体 Polyclonal Antibody to Hairy And Enhancer Of Split 1 (HES1)

PAJ705Mi01 H2A组蛋白家族成员Z(H2AFZ)多克隆抗体 Polyclonal Antibody to H2A Histone Family, Member Z (H2AFZ)

PAJ707Hu01 H2A组蛋白家族成员Y(H2AFY)多克隆抗体 Polyclonal Antibody to H2A Histone Family, Member Y (H2AFY)

PAQ000Hu01 H2A组蛋白家族成员X(H2AFX)多克隆抗体 Polyclonal Antibody to H2A Histone Family, Member X (H2AFX)

PAJ708Hu01 H2A组蛋白家族成员V(H2AFV)多克隆抗体 Polyclonal Antibody to H2A Histone Family, Member V (H2AFV)

PAS779Hu01 H2A组蛋白家族成员J(H2AFJ)多克隆抗体 Polyclonal Antibody to H2A Histone Family, Member J (H2AFJ)

PAG292Hu01 鸟苷酸环化酶激活因子2B(GUCA2B)多克隆抗体 Polyclonal Antibody to Guanylate Cyclase Activator 2B (GUCA2B)

PAG291Hu01 鸟苷酸环化酶激活因子2A(GUCA2A)多克隆抗体 Polyclonal Antibody to Guanylate Cyclase Activator 2A (GUCA2A)

PAG291Mu01 鸟苷酸环化酶激活因子2A(GUCA2A)多克隆抗体 Polyclonal Antibody to Guanylate Cyclase Activator 2A (GUCA2A)

PAG291Mu02 鸟苷酸环化酶激活因子2A(GUCA2A)多克隆抗体 Polyclonal Antibody to Guanylate Cyclase Activator 2A (GUCA2A)

PAH947Hu01 鸟苷酸环化酶1β3(GUCY1β3)多克隆抗体 Polyclonal Antibody to Guanylate Cyclase 1 Beta 3 (GUCY1b3)

PAJ051Hu01 鸟嘌呤脱氨酶有丝分裂阻滞缺陷2样蛋白2抗体(GDA)多克隆抗体 Polyclonal Antibody to Guanine Deaminase (GDA)

PAB604Hu01 生长调节致癌基因γ(GROγ)多克隆抗体 Polyclonal Antibody to Growth Regulated Oncogene Gamma (GROg)

PAB604Ra01 生长调节致癌基因γ(GROγ)多克隆抗体 Polyclonal Antibody to Growth Regulated Oncogene Gamma (GROg)

PAB604Hu02 生长调节致癌基因γ(GROγ)多克隆抗体 Polyclonal Antibody to Growth Regulated Oncogene Gamma (GROg)

PAB603Hu01 生长调节致癌基因β(GROβ)多克隆抗体 Polyclonal Antibody to Growth Regulated Oncogene Beta (GROb)

PAB603Hu02 生长调节致癌基因β(GROβ)多克隆抗体 Polyclonal Antibody to Growth Regulated Oncogene Beta (GROb)