膜蛋白相互作用蛋白RGS16抗体
『别名』EC 3.1.4.44; GDE1; Glycerophosphodiester phosphodiesterase 1; Membrane interacting protein of RGS16; RGS16 interacting membrane protein; GDE1_HUMAN.
『浓度』膜蛋白相互作用蛋白RGS16抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
膜蛋白相互作用蛋白RGS16抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
膜蛋白相互作用蛋白RGS16抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAJ786Mu02 羟烷基辅酶A脱氢酶β(HADHβ)多克隆抗体 Polyclonal Antibody to Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb)
PAG364Ra01 羟酸氧化酶1(HAO1)多克隆抗体 Polyclonal Antibody to Hydroxyacid Oxidase 1 (HAO1)
PAG364Ra02 羟酸氧化酶1(HAO1)多克隆抗体 Polyclonal Antibody to Hydroxyacid Oxidase 1 (HAO1)
PAG364Mu01 羟酸氧化酶1(HAO1)多克隆抗体 Polyclonal Antibody to Hydroxyacid Oxidase 1 (HAO1)
PAH843Hu01 氢离子电压门控通道蛋白1(HVCN1)多克隆抗体 Polyclonal Antibody to Hydrogen Voltage Gated Channel Protein 1 (HVCN1)
PAE237Mu01 透明质酸氨基葡糖苷酶2(HYAL2)多克隆抗体 Polyclonal Antibody to Hyaluronoglucosaminidase 2 (HYAL2)
PAS092Hu01 透明质酸氨基葡糖苷酶1(HYAL1)多克隆抗体 Polyclonal Antibody to Hyaluronoglucosaminidase 1 (HYAL1)
PAS092Mu01 透明质酸氨基葡糖苷酶1(HYAL1)多克隆抗体 Polyclonal Antibody to Hyaluronoglucosaminidase 1 (HYAL1)
PAS092Ra01 透明质酸氨基葡糖苷酶1(HYAL1)多克隆抗体 Polyclonal Antibody to Hyaluronoglucosaminidase 1 (HYAL1)
PAB217Hu01 透明质酸酶(HAase)多克隆抗体 Polyclonal Antibody to Hyaluronidase (HAase)
PAB217Mu01 透明质酸酶(HAase)多克隆抗体 Polyclonal Antibody to Hyaluronidase (HAase)
PAB217Ra01 透明质酸酶(HAase)多克隆抗体 Polyclonal Antibody to Hyaluronidase (HAase)
PAA182Ge01 透明质酸(HA)多克隆抗体 Polyclonal Antibody to Hyaluronic Acid (HA)
PAF472Hu01 透明质酸合酶2(HAS2)多克隆抗体 Polyclonal Antibody to Hyaluronan Synthase 2 (HAS2)
PAC520Hu01 透明质酸合酶1(HAS1)多克隆抗体 Polyclonal Antibody to Hyaluronan Synthase 1 (HAS1)
PAC520Ra01 透明质酸合酶1(HAS1)多克隆抗体 Polyclonal Antibody to Hyaluronan Synthase 1 (HAS1)
PAC065Hu01 透明质酸介导运动因子受体(HMMR)多克隆抗体 Polyclonal Antibody to Hyaluronan Mediated Motility Receptor (HMMR)
PAC517Hu01 透明质酸结合蛋白2(HABP2)多克隆抗体 Polyclonal Antibody to Hyaluronan Binding Protein 2 (HABP2)
PAA651Hu01 透明质酸结合蛋白1(HABP1)多克隆抗体 Polyclonal Antibody to Hyaluronan Binding Protein 1 (HABP1)
PAA651Mu01 透明质酸结合蛋白1(HABP1)多克隆抗体 Polyclonal Antibody to Hyaluronan Binding Protein 1 (HABP1)
PAA651Ra01 透明质酸结合蛋白1(HABP1)多克隆抗体 Polyclonal Antibody to Hyaluronan Binding Protein 1 (HABP1)
PAA651Bo01 透明质酸结合蛋白1(HABP1)多克隆抗体 Polyclonal Antibody to Hyaluronan Binding Protein 1 (HABP1)
PAC525Hu01 亨廷顿相互作用蛋白12(HIP12)多克隆抗体 Polyclonal Antibody to Huntingtin Interacting Protein 12 (HIP12)
PAH923Hu01 亨廷顿关联蛋白1(HAP1)多克隆抗体 Polyclonal Antibody to Huntingtin Associated Protein 1 (HAP1)
PAH923Mu01 亨廷顿关联蛋白1(HAP1)多克隆抗体 Polyclonal Antibody to Huntingtin Associated Protein 1 (HAP1)
PAH922Hu01 亨廷顿蛋白(HTT)多克隆抗体 Polyclonal Antibody to Huntingtin (HTT)
PAH922Mu01 亨廷顿蛋白(HTT)多克隆抗体 Polyclonal Antibody to Huntingtin (HTT)
PAH922Ra01 亨廷顿蛋白(HTT)多克隆抗体 Polyclonal Antibody to Huntingtin (HTT)
PAH922Mu02 亨廷顿蛋白(HTT)多克隆抗体 Polyclonal Antibody to Huntingtin (HTT)
PAN048Hu01 HtrA丝氨酸肽酶4(HTRA4)多克隆抗体 Polyclonal Antibody to HtrA Serine Peptidase 4 (HTRA4)
PAL604Hu01 HtrA丝氨酸肽酶1(HTRA1)多克隆抗体 Polyclonal Antibody to HtrA Serine Peptidase 1 (HTRA1)
PAL604Co01 HtrA丝氨酸肽酶1(HTRA1)多克隆抗体 Polyclonal Antibody to HtrA Serine Peptidase 1 (HTRA1)
PAL604Co02 HtrA丝氨酸肽酶1(HTRA1)多克隆抗体 Polyclonal Antibody to HtrA Serine Peptidase 1 (HTRA1)
PAH884Hu01 Hornerin蛋白(HRNR)多克隆抗体 Polyclonal Antibody to Hornerin (HRNR)
PAD717Ge01 高香草酸(HVA)多克隆抗体 Polyclonal Antibody to Homovanillic Acid (HVA)
PAE250Hu01 尿黑酸1,2-双加氧酶(HGD)多克隆抗体 Polyclonal Antibody to Homogentisate-1,2-Dioxygenase (HGD)
PAE250Hu02 尿黑酸1,2-双加氧酶(HGD)多克隆抗体 Polyclonal Antibody to Homogentisate-1,2-Dioxygenase (HGD)
PAE250Mu01 尿黑酸1,2-双加氧酶(HGD)多克隆抗体 Polyclonal Antibody to Homogentisate-1,2-Dioxygenase (HGD)
PAD984Ge01 高半胱氨酸(HCy)多克隆抗体 Polyclonal Antibody to Homocysteine (HCy)
PAA670Mu01 归巢关联细胞黏附分子(HCAM)多克隆抗体 Polyclonal Antibody to Homing Associated Cell Adhesion Molecule (HCAM)
PAA670Mu02 归巢关联细胞黏附分子(HCAM)多克隆抗体 Polyclonal Antibody to Homing Associated Cell Adhesion Molecule (HCAM)
PAA670Ra01 归巢关联细胞黏附分子(HCAM)多克隆抗体 Polyclonal Antibody to Homing Associated Cell Adhesion Molecule (HCAM)
PAA670Hu01 归巢关联细胞黏附分子(HCAM)多克隆抗体 Polyclonal Antibody to Homing Associated Cell Adhesion Molecule (HCAM)
PAF477Hu01 同源域相互作用蛋白激酶2(HIPK2)多克隆抗体 Polyclonal Antibody to Homeodomain Interacting Protein Kinase 2 (HIPK2)
PAD310Hu01 全细胞色素C合酶(HCCS)多克隆抗体 Polyclonal Antibody to Holocytochrome C Synthase (HCCS)
PAJ947Mu01 HLA-B关联转录因子3(BAT3)多克隆抗体 Polyclonal Antibody to HLA-B Associated Transcript 3 (BAT3)
PAJ947Mu02 HLA-B关联转录因子3(BAT3)多克隆抗体 Polyclonal Antibody to HLA-B Associated Transcript 3 (BAT3)
PAA289Mi01 组蛋白H4(H4)多克隆抗体 Polyclonal Antibody to Histone H4 (H4)
PAA289Mi02 组蛋白H4(H4)多克隆抗体 Polyclonal Antibody to Histone H4 (H4)
PAA285Mi01 组蛋白H3(H3)多克隆抗体 Polyclonal Antibody to Histone H3 (H3)
PAA285Mi02 组蛋白H3(H3)多克隆抗体 Polyclonal Antibody to Histone H3 (H3)
PAE909Hu01 组蛋白脱乙酰基酶9(HDAC9)多克隆抗体 Polyclonal Antibody to Histone Deacetylase 9 (HDAC9)
PAE906Hu01 组蛋白脱乙酰基酶6膜蛋白相互作用蛋白RGS16抗体(HDAC6)多克隆抗体 Polyclonal Antibody to Histone Deacetylase 6 (HDAC6)
PAE906Hu02 组蛋白脱乙酰基酶6(HDAC6)多克隆抗体 Polyclonal Antibody to Histone Deacetylase 6 (HDAC6)
PAE906Ra01 组蛋白脱乙酰基酶6(HDAC6)多克隆抗体 Polyclonal Antibody to Histone Deacetylase 6 (HDAC6)
PAE906Mu01 组蛋白脱乙酰基酶6(HDAC6)多克隆抗体 Polyclonal Antibody to Histone Deacetylase 6 (HDAC6)