阿拉伯聚糖检测试剂盒

产品货号：ZZK-K-ARAB

产品品名：阿拉伯聚糖检测试剂盒

英文品名：Arabinan Assay Kit

规格型号：100 assays per kit  

For the measurement of arabinan in fruit juice concentrates.

INTRODUCTION:

In the processing of apples and pears, the yield of juice can be

dramatically improved by using enzymes to degrade the pulp

polysaccharides, together with more exhaustive extraction of the

pulp with diffusion equipment. Of course, these processes also

significantly increase the amount of partially degraded polysaccharide

which is solubilised. This polysaccharide material may be soluble as

extracted, but subsequent changes in temperature and pH conditions

can directly lead to precipitation (or crystallisation) or to chemical

modification followed by precipitation. Such a problem can be

experienced in the production of clear apple or pear juice, in which

case, an arabinan haze material is produced. This material was shown

to be microcrystalline 1,5-α-L-arabinan.

Arabinans, as present in cell-wall pectic-substances, have been shown

to consist of a main chain of 1,5-α-linked L-arabinofuranosyl residues

to which other L-arabinofuranosyl residues are linked 1,3-α and 1,2-α

in either a comb-like or a ramified arrangement.

It is generally accepted that the best solution to this “arabinan

haze” problem is to use pectinase enzyme preparations containing

high levels of both α-L-arabinofuranosidase and endo-1,5-α-Larabinanase.

The combined action of these two enzymes reduces

arabinan to arabinose and lower degree of polymerisation, arabinooligosaccharides.

KITS:

Kits suitable for performing 100 assays are available from Megazyme.

The kits contain the full assay method plus:

Bottle 1: Buffer (25 mL, pH 8.6) plus sodium azide (0.02 %

w/v) as a preservative (Buffer A).

Stable for > 2 years at 4°C.

Bottle 2: NAD+.

Stable for > 5 years at -20°C.

Bottle 3: α-L-Arabinofuranosidase plus endo-arabinanase

suspension (2.2 mL).

Stable for > 4 years at 4°C.

Bottle 4: β-Galactose dehydrogenase plus galactose mutarotase

suspension, 2.2 mL.

Stable for > 2 years at 4°C.

Bottle 5: L-Arabinose standard solution (5 mL, 0.5 mg/mL in

0.02 % sodium azide).

Stable for > 4 years at 4°C.

Bottle 6: Arabinan control in mannitol: Arabinan

concentration on vial.

Stable for > 5 years at 4°C.

Component 7: Pharmacia PD-10 column.

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