L-阿拉伯糖/D-半乳糖检测试剂盒

产品货号：ZZK-K-ARGA

产品品名：L-阿拉伯糖/D-半乳糖检测试剂盒

英文品名：L-Arabinose / D-Galactose Assay Kit

规格型号：115 assays (manual) / 1150 assays (microplate) / 1150 assays (auto-analyser)

A simple, reliable and accurate UV method for the measurement ofL-arabinose and/or D-galactose in various materials including foods,feeds, beverage and plant products.

INTRODUCTION:

Enzymic kits for the determination of L-arabinose and D-galactose

are very slow. This is due to the low rate of natural chemical

“mutarotation” between the α- and β-anomeric forms of these

monosaccharides. Only the β-form is recognised by β-galactose

dehydrogenase. In incubations containing NAD+, L-arabinose

or D-galactose and β-galactose dehydrogenase, there is a very

rapid initial increase in absorbance due to the consumption of β

-L-arabinose or β-D-galactose, and this is followed by a very slow

approach to the end-point. This very slow approach results from

the very low rate of chemical “mutarotation” of the α-anomeric

conformation of these monosaccharides to the respective β-anomer.

Using technology developed by Megazyme (patent pending), a

galactose mutarotase has now been incorporated into the assay

format to rapidly catalyse this rate-limiting mutarotation step.

The result is very rapid analysis times of approximately 6 min for

D-galactose and approximately 12 min for L-arabinose (Figure 1).

KITS:

Kits suitable for performing 115 assays in manual format (or 1150 assays

in microplate or auto-analyser formats) are available from Megazyme.

The kits contain the full assay method plus:

Bottle 1: Buffer (25 mL, pH 8.6) plus sodium azide (0.02% w/v) as

a preservative. Stable for > 2 years at 4°C.

Bottle 2: NAD+. Lyophilised powder.

Stable for > 5 years at -20°C.

Bottle 3: β-Galactose dehydrogenase plus galactose mutarotase

suspension, 2.4 mL. Stable for > 2 years at 4°C.

Bottle 4: L-Arabinose standard solution (5 mL, 0.4 mg/mL in

0.02% w/v sodium azide).

Stable for > 2 years at 4°C.

Bottle 5: D-Galactose standard solution (5 mL, 0.4 mg/mL in

0.02% w/v sodium azide).

Stable for > 2 years at 4°C.

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