柠檬酸检测试剂盒

产品货号：ZZK-K-CITR

产品品名：柠檬酸检测试剂盒

英文品名：Citric Acid Assay Kit

规格型号：72 assays (manual) / 720 assays (microplate) / 840 assays (auto-analyser)

A flexible and simple method for the rapid and reliable measurement ofcitric acid (citrate) in foods, beverages and other materials.

INTRODUCTION:

Due to its excellent acidulant, flavorant and preservative properties,

citric acid (citrate) is found in a large number of natural and

processed foods and beverages, such as fruit juice and other soft

drinks, beer, milk, bread, candies, and dairy and meat products. This

acid also finds many other applications, such as in paper manufacture,

or in the wine industry, where the presence of significant quantities

indicates the use of citric acid as an acidulant, a practice with an

allowable upper limit of just 1 g/L (final concentration) in the EU.

The quantification of citric acid is also important in clinical chemistry.

Polyvinylpyrollidone (PVP) has been incorporated into the Megazyme

assay format to prevent inhibition caused by tannins found in grape

juice, fermenting must and wine. In addition to a > 2 years shelf life

and competitive price, manual (see page 5 “A”), auto-analyser (see

page 6 “B”) and microplate (see page 8 “C”) assay procedures are

described, making this product ideal for citric acid determination

applications in laboratories of any size.

KITS:

Kits suitable for performing 72 assays in manual format (or 840

assays in auto-analyser format or 720 assays in microplate format) are

available from Megazyme. The kits contain the full assay method plus:

Bottle 1: Buffer (40 mL, pH 7.5) plus sodium azide (0.02%) as

a preservative. Stable for > 2 years at 4°C.

Bottle 2: NADH plus PVP.

Stable for > 5 years at -20°C.

Bottle 3: L-Malate dehydrogenase plus D-lactate

dehydrogenase, 1.5 mL.

Stable for > 2 years at 4°C.

Bottle 4: (x 3) Citrate lyase lyophilisate.

Stable for > 2 years at -20°C.

Bottle 5: Citric acid standard solution (5 mL, 0.20 mg/mL) in

0.02% (w/v) sodium azide.

Stable for > 2 years at 4°C.

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