人Ⅳ型胶原(COL4)ELISA试剂盒

人Ⅳ型胶原(COL4)ELISA试剂盒试剂

人Ⅳ型胶原(COL4)ELISA试剂盒实验ABTS使用液:

ABTS　　　　　　　　0.5mg

底物缓冲液(PH5.5)　 1ml

3%H2O2 2μl

人Ⅳ型胶原(COL4)ELISA试剂盒实验封闭液：

牛血清白蛋白(BSA) 5克

加洗涤缓冲液至100ml。

值得一提的是有的封闭液中含有脱脂奶粉，不适合长期保存。

人Ⅳ型胶原(COL4)ELISA试剂盒板应该是吸附性能好，空白值低，孔底透明度高，各板之间、同一板各孔之间、同一板各孔之间性能相近。聚苯乙烯ELISA板由于原料的不同和制作工艺的差别，各种产品的质量差异很大，因此，每一批号的ELISA板在使用前须事先检查其性能。常用的检查方法为：以一定浓度的人IgG(一般为10ng/ml)包被人Ⅳ型胶原(COL4)ELISA试剂盒板各孔，洗涤后每孔内加入适当稀释度的酶标抗人IgG抗体，保温后洗涤，加底物显色，终止酶反应后，分别测每孔溶液的吸光度。控制反应条件，使各孔读数在吸光度0.8左右。计算全部读数的平均值。所有单个读数与全部读数的均数之差，应小于10%。

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