人糖原合酶激酶3α(GSK3α)ELISA试剂盒原理
辣根过氧化物酶 (horse radish peroxidase,简称HRP,EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶,早在20世纪30年代就有人着手从辣根中分离此酶,以后又制备出结晶。本实验是以辣根为原料,经过水的抽提,硫酸铵和丙酮分级分离,再经锌离子纯化,透析除盐,冰冻干燥,便可得到高纯度的辣根过氧化物酶。
人糖原合酶激酶3α(GSK3α)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质,HRP广泛分布于植物界,它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白,糖含量18%。HRP由多个同功酶组成,Mr在40000左右,等电点7.2。溶于水,溶解度为5%(W/V),溶液呈棕红色,透明。人糖原合酶激酶3α(GSK3α)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异,但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液,而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下,HRP几周内稳定,加热到63℃,在15min内稳定。人糖原合酶激酶3α(GSK3α)ELISA试剂盒氧化还原电势很低,pH6.08时,E 0 =-0.2070V,pH为7.71时,E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm,一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b
酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否,因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体,它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体,其次要有良好的制备方法。目前,高质量的酶(如辣根过氧化物酶,简称HRP)国内已有商品供应。人糖原合酶激酶3α(GSK3α)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上,宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。
XY-EL-P0675c 猪高尔基蛋白73(GP-73)ELISA试剂盒 Porcine GP-73 (Golgi Protein 73) ELISA Kit
XY-EL-P0677c 猪染色质区解旋酶DNA结合蛋白3(CHD3)ELISA试剂盒 Porcine CHD3 (Chromodomain Helicase DNA Binding Protein 3) ELISA Kit
XY-EL-P0679c 猪内凝集蛋白1(ITLN1)ELISA试剂盒 Porcine ITLN1 (Intelectin 1/Omentin) ELISA Kit
XY-EL-P0680c 猪基质细胞衍生因子1(SDF-1/CXCL12)ELISA试剂盒 Porcine SDF-1/CXCL12 (Stromal Cell Derived Factor 1) ELISA Kit
XY-EL-P0681c 猪内质网核信号1(ERN1)ELISA试剂盒 Porcine ERN1 (Endoplasmic Reticulum to Nucleus Signalling 1) ELISA Kit
XY-EL-P0682c 猪硫氧还蛋白结合蛋白2(TBP2)ELISA试剂盒 Porcine TBP2 (Thioredoxin Binding Protein 2) ELISA Kit
XY-EL-P0683c 猪血小板亲和蛋白2(PKP2)ELISA试剂盒 Porcine PKP2 (Plakophilin 2) ELISA Kit
XY-EL-P0684c 猪巨噬细胞趋化因子(MCF)ELISA试剂盒 Porcine MCF (Macrophage Chemotatic Factor) ELISA Kit
XY-EL-P0685c 猪G补缀FHA域血管生成因子1(AGGF1)ELISA试剂盒 Porcine AGGF1 (Angiogenic Factor with G Patch and FHA Domains 1) ELISA Kit
XY-EL-P0686c 猪谷胱甘肽S转移酶ω1(GSTω1)ELISA试剂盒 Porcine GSTω1 (Glutathione S Transferase Omega 1) ELISA Kit
XY-EL-P0687c 猪免疫球蛋白G Fc段结合蛋白(FcγBP)ELISA试剂盒 Porcine FcγBP (Fc Fragment of IgG Binding Protein) ELISA Kit
XY-EL-P0689c 猪VII型胶原(COL7)ELISA试剂盒 Porcine COL7 (Collagen Type VII) ELISA Kit
XY-EL-P0690c 猪血小板衍生生长因子BB(PDGF-BB)ELISA试剂盒 Porcine PDGF-BB (Platelet Derived Growth Factor-BB) ELISA Kit
XY-EL-P0691c 猪胸腺激活调节趋化因子(TARC)ELISA试剂盒 Porcine TARC (Thymus Activation Regulated Chemokine) ELISA Kit
XY-EL-P0693c 猪肿瘤坏死因子受体超家族成员1B(TNFRSF1B)ELISA试剂盒 Porcine TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) ELISA Kit
XY-EL-P0694c 猪T细胞特异性鸟苷三磷酸酶(Tgtp)ELISA试剂盒 Porcine Tgtp (T-cell Specific Gtpase) ELISA Kit
XY-EL-P0695c 猪上皮膜抗原(EMA)ELISA试剂盒 Porcine EMA (Epithelial Membrane Antigen) ELISA Kit
XY-EL-P0696c 猪结蛋白(Des)ELISA试剂盒 Porcine Des (Desmin) ELISA Kit
XY-EL-P0697c 猪乳酸脱氢酶C(LDHC)ELISA试剂盒 Porcine LDHC (Lactate Dehydrogenase C) ELISA Kit
XY-EL-P0698c 猪非受体酪氨酸激酶2(TNK2)ELISA试剂盒 Porcine TNK2 (Tyrosine Kinase, Non Receptor 2) ELISA Kit
XY-EL-P0699c 猪黑色素瘤转移表面黏附分子(MMSAM)ELISA试剂盒 Porcine MMSAM (Melanoma Metastasis Surface Adhesion Molecule) ELISA Kit
XY-EL-P0700c 猪表皮脂肪酸结合蛋白5(FABP5)ELISA试剂盒 Porcine FABP5 (Fatty Acid Binding Protein 5, Epidermal ) ELISA Kit
XY-EL-P0701c 猪胃内因子(GIF)ELISA试剂盒 Porcine GIF (Gastric Intrinsic Factor) ELISA Kit
XY-EL-P0703c 猪脱氢表雄酮硫酸酯(DHEA-S)ELISA试剂盒 Porcine DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit
XY-EL-P0704c 猪乳酸脱氢酶B(LDHB)ELISA试剂盒 Porcine LDHB (Lactate Dehydrogenase B) ELISA Kit
XY-EL-P0705c 猪D-乳酸脱氢酶(D-LDH)ELISA试剂盒 Porcine D-LDH (D-Lactate Dehydrogenase) ELISA Kit
XY-EL-P0706c 猪芳基硫酸酯酶B(ASB)ELISA试剂盒 Porcine ASB (Arylsulfatase B) ELISA Kit