人补体成分5a(C5a)ELISA试剂盒

人补体成分5a(C5a)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人补体成分5a(C5a)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人补体成分5a(C5a)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人补体成分5a(C5a)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人补体成分5a(C5a)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

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XY-EL-M1021c 小鼠核糖核苷酸酶T2(RNASET2)ELISA试剂盒 Mouse RNASET2 (Ribonuclease T2) ELISA Kit  

XY-EL-M1022c 小鼠核糖体蛋白L10(RPL10)ELISA试剂盒 Mouse RPL10 (Ribosomal Protein L10) ELISA Kit  

XY-EL-M1023c 小鼠核糖体蛋白L26(RPL26)ELISA试剂盒 Mouse RPL26 (Ribosomal Protein L26) ELISA Kit  

XY-EL-M1024c 小鼠环指蛋白4(RNF4)ELISA试剂盒 Mouse RNF4 (Ring Finger Protein 4) ELISA Kit  

XY-EL-M1030c 小鼠Runt相关转录因子2(RUNX2)ELISA试剂盒 Mouse RUNX2 (Runt Related Transcription Factor 2) ELISA Kit 

XY-EL-M1031c 小鼠钙结合蛋白S100A11(S100A11) ELISA试剂盒 Mouse S100A11 (S100 Calcium Binding Protein A11) ELISA Kit   

XY-EL-M1032c 小鼠钙结合蛋白S100A7(S100A7) ELISA试剂盒 Mouse S100A7 (S100 Calcium Binding Protein A7) ELISA Kit  

XY-EL-M1033c 小鼠S100钙结合蛋白B (S100B) ELISA试剂盒 Mouse S100B (S100 Calcium Binding Protein B) ELISA Kit  

XY-EL-M1035c 小鼠钙结合蛋白S100A6(S100A6) ELISA试剂盒 Mouse S100A6 (S100 Calcium Binding Protein A6) ELISA Kit

XY-EL-M1036c 小鼠S100钙结合蛋白A12(S100A12 )ELISA试剂盒 Mouse S100A12 (S100 Calcium Binding Protein A12) ELISA Kit

XY-EL-M1037c 小鼠山梨醇脱氢酶(SDH)ELISA试剂盒 Mouse SDH (Sorbitol Dehydrogenase) ELISA Kit

XY-EL-M1038c 小鼠分泌型磷脂酶A2(sPLA2)ELISA试剂盒 Mouse sPLA2 (Screted Phospholipase A2) ELISA Kit 

XY-EL-M1039c 小鼠促胰液素(SCT)ELISA试剂盒 Mouse SCT (Secretin) ELISA Kit  

XY-EL-M1040c 小鼠分泌型免疫球蛋白A(sIgA)ELISA试剂盒 Mouse sIgA (Secretory Immunoglobulin A) ELISA Kit

XY-EL-M1042c 小鼠硒蛋白P(SEPP1)ELISA试剂盒 Mouse SEPP1 (Selenoprotein P) ELISA Kit 

XY-EL-M1043c 小鼠脑信号素7A(SEMA7A)ELISA试剂盒 Mouse SEMA7A (Semaphorin 7A) ELISA Kit  

XY-EL-M1045c 小鼠丝氨酸/苏氨酸蛋白磷酸酶(STK)ELISA试剂盒 Mouse STK (serine/threonine protein kinase) ELISA Kit

XY-EL-M1046c 小鼠血清淀粉样蛋白A(SAA)ELISA试剂盒 Mouse SAA (Serum amyloid A) ELISA Kit

XY-EL-M1047c 小鼠Sestrin 1蛋白 (SESN1) ELISA试剂盒 Mouse SESN1 (Sestrin 1) ELISA Kit  

XY-EL-M1048c 小鼠Sestrin 2蛋白 (SESN2) ELISA试剂盒 Mouse SESN2 (Sestrin 2) ELISA Kit 

XY-EL-M1049c 小鼠Sestrin 3蛋白 (SESN3) ELISA试剂盒 Mouse SESN3 (Sestrin 3) ELISA Kit  

XY-EL-M1050c 小鼠性别决定区Y框蛋白3(SOX3) ELISA试剂盒 Mouse SOX3 (Sex Determining Region Y Box Protein 3) ELISA Kit  

XY-EL-M1051c 小鼠性激素结合球蛋白(SHBG)ELISA试剂盒 Mouse SHBG (Sex Hormone-Binding Globulin) ELISA Kit

XY-EL-M1052c 小鼠唾液酸结合免疫球蛋白样凝集素3(SIGLEC3) ELISA试剂盒 Mouse SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3) ELISA Kit  

XY-EL-M1053c 小鼠唾液酸结合免疫球蛋白样凝集素12(SIGLEC12)ELISA试剂盒 Mouse SIGLEC12 (Sialic Acid Binding Ig Like Lectin 12) ELISA Kit  

XY-EL-M1054c 小鼠信号识别颗粒(SRP10)ELISA试剂盒 Mouse SRP10 (Signal Recognition Particle 9kDa) ELISA Kit  

XY-EL-M1055c 小鼠信号转导子和转录激活子1(STAT1)ELISA试剂盒 Mouse STAT1 (Signal Transducer And Activator Of Transcription 1) ELISA Kit 

XY-EL-M1056c 小鼠信号转导子和转录激活子2(STAT2)ELISA试剂盒 Mouse STAT2 (Signal Transducer And Activator Of Transcription 2) ELISA Kit  

XY-EL-M1057c 小鼠信号转导子和转录激活子3(STAT3)ELISA试剂盒 Mouse STAT3 (Signal Transducer And Activator Of Transcription 3) ELISA Kit  

XY-EL-M1058c 小鼠信号转导子和转录激活子4(STAT4)ELISA试剂盒 Mouse STAT4 (Signal Transducer And Activator Of Transcription 4) ELISA Kit  

XY-EL-M1059c 小鼠信号转导子和转录激活子5A(STAT5A)ELISA试剂盒 Mouse STAT5A (Signal Transducer And Activator Of Transcription 5A) ELISA Kit  

XY-EL-M1060c 小鼠信号转导子和转录激活子6B(STAT6B)ELISA试剂盒 Mouse STAT6B (Signal Transducer And Activator Of Transcription 5B) ELISA Kit  

XY-EL-M1061c 小鼠信号转导子和转录激活子6(STAT6)ELISA试剂盒 Mouse STAT6 (Signal Transducer And Activator Of Transcription 6) ELISA Kit  

XY-EL-M1062c 小鼠Smad同源物1(Smad1)ELISA试剂盒 Mouse Smad1 (Mothers Against Decapentaplegic Homolog 1) ELISA Kit

XY-EL-M1063c 小鼠鸟氨酸氨基甲酰转移酶(OCT)ELISA试剂盒 Mouse OCT (Ornithine Carbamoyl Transferase) ELISA Kit

XY-EL-M1064c 小鼠辅肌动蛋白α2 (ACTN2)ELISA试剂盒 Mouse ACTN2 (Actinin Alpha 2) ELISA Kit