人脂肪分化相关蛋白(ADRP)ELISA试剂盒

人脂肪分化相关蛋白(ADRP)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人脂肪分化相关蛋白(ADRP)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人脂肪分化相关蛋白(ADRP)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人脂肪分化相关蛋白(ADRP)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人脂肪分化相关蛋白(ADRP)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-H2583c 人鞘氨醇-1-磷酸(S1P)ELISA试剂盒 Human S1P (Sphingosine 1 phosphate) ELISA Kit

XY-EL-H2584c 人可溶性尿激酶型纤溶酶原激活因子受体(suPAR)ELISA试剂盒 Human suPAR(soluble urokinase-type plasminogen activator receptor) ELISA Kit

XY-EL-H2585c 人细胞间粘附分子1(ICAM-1/CD54)ELISA试剂盒 Human ICAM-1 (intercellular adhesion molecule 1) ELISA Kit 

XY-EL-H4184c 人孕激素相关子宫内膜蛋白(PAEP)ELISA试剂盒 Human PAEP (Progestagen-Associated Endometrial Protein) ELISA Kit

XY-EL-H5022c 人白介素11(IL-11)ELISA试剂盒 Human IL-11 (Interleukin 11) ELISA Kit

XY-EL-H5105c 人小而密低密度脂蛋白(sdLDL)ELISA试剂盒 Human sdLDL (Small Dense Low Density Lipoprotein) ELISA Kit

XY-EL-H5134c 人高敏C反应蛋白(CRP)ELISA试剂盒 Human hs-CRP (high-sensitivity C-reactive protein) ELISA Kit

XY-EL-H5135c 人促甲状腺素受体(TSHR)ELISA试剂盒 Human TSHR (Thyroid Stimulating Hormone Receptor) ELISA Kit 

XY-EL-H5200c 人乳铁传递蛋白(LTF)检测试剂盒(酶联免疫吸附试验法) Human Lactoferrin (LTF) ELISA Kit

XY-EL-H5300c 人端粒重复结合因子2相互作用蛋白(TERF2IP)ELISA试剂盒 Human TERF2IP (Telomeric Repeat Binding Factor 2 Interacting Protein) ELISA Kit

XY-EL-H5301c 人含Ⅲ型纤连蛋白域蛋白5(FNDC5)ELISA试剂盒 Human FNDC5 (Fibronectin Type III Domain Containing Protein 5 ) ELISA Kit

XY-EL-H5302c 人抗双链DNA抗体(Anti-dsDNA)ELISA试剂盒 Human Anti-dsDNA (Anti-Double Stranded DNA Antibody) ELISA Kit

XY-EL-H5303c 人类二氢硫辛胺支链转酰基酶E2(DBT)ELISA试剂盒 Human DBT (Dihydrolipoamide branched chain transacylase E2) ELISA kit

XY-EL-H5307c 人Adropin蛋白(AD)ELISA试剂盒 Human AD (Adropin) ELISA kit

XY-EL-H5308c 人Salusin β蛋白(Salusin β)ELISA试剂盒 Human Salusin β (Salusin Beta) ELISA Kit

XY-EL-H5309c 人可溶性内皮糖蛋白(ENG)ELISA试剂盒 Human ENG (Soluble Endoglin) ELISA Kit

XY-EL-H5310c 人肝细胞生长因子受体(c-MET/HGFR)ELISA试剂盒 Human c-MET/HGFR (hepatocyte growth factor receptor) ELISA Kit

XY-EL-H5311c 人RECK蛋白(RECK)ELISA试剂盒 Human RECK (Reversion-inducing-cysteine-rich protein with kazal motifs) ELISA Kit

XY-EL-H5312c 人CD44变体5(CD44v5)ELISA试剂盒 Human CD44v5 (cluster of differentiation 44 variant 5)ELISA Kit

XY-EL-H5313c 人色胺酸羟化酶1(TPH1)ELISA试剂盒 Human Tryptophan Hydroxylase 1 (TPH1) ELISA Kit

XY-EL-H5314c 人磷酸化Tau蛋白(pMAPT/pTAU)ELISA kit Human pMAPT /pTAU (phosphorylated microtubule-associated protein tau) ELISA kit

XY-EL-H5315c 人类17-β-羟基类固醇脱氢酶10(HSD17β10)ELISA试剂盒 Human HSD17b10 (17-Beta-Hydroxysteroid Dehydrogenase Type 10) ELISA Kit

XY-EL-H5316c 人组蛋白脱乙酰基酶1(HDAC1)ELISA试剂盒 Human HDAC1 (Histone Deacetylase 1) ELISA Kit

XY-EL-H5317c 人组织相容性复合体Ⅰ类相关基因A(MICA)ELISA试剂盒 Human MICA (major histocompatibility complex class I chain-related gene A) ELISA Kit

XY-EL-H5400c 人血管内皮细胞生长因子(VEGF)ELISA试剂盒 Human VEGF(Vascular Endothelial cell Growth Factor) ELISA KIT

XY-EL-H5401c 人吻素(KISS)ELISA试剂盒 Human KISS(Kisspeptin)ELISA KIT

XY-EL-H5402c 人内皮糖蛋白(ENG)检测试剂盒 Human ENG(Endoglin)ELISA Kit 

XY-EL-H5405c 人信号肽，CUB域，EGF样1 (SCUBE1)ELISA试剂盒 Human SCUBE1 (Signal Peptide, CUB and EGF-like Domain-containing Protein 1)ELISA Kit

XY-EL-H5406c 人一氧化氮(NO)ELISA试剂盒   Human NO(nitric oxide) ELISA Kit

XY-EL-H5407c 人氧化型谷胱甘肽(GSSG)ELISA试剂盒 Human GSSG(Oxidizided Glutathione) ELISA Kit

XY-EL-H5408c 人过氧化氢酶(CAT)ELISA kit Human CAT(Catalase) ELISA kit

XY-EL-H5409c 人总胆汁酸(TBA)ELISA试剂盒 Human TBA(Total bile?acid)?kit