人髓鞘碱性蛋白(MBP)ELISA试剂盒

人髓鞘碱性蛋白(MBP)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人髓鞘碱性蛋白(MBP)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人髓鞘碱性蛋白(MBP)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人髓鞘碱性蛋白(MBP)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人髓鞘碱性蛋白(MBP)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-H1563c 人蛋白二硫化物异构酶A6(PDIA6)ELISA试剂盒 Human PDIA6 (Protein Disulfide Isomerase A6) ELISA Kit

XY-EL-H1564c 人红细胞衍生核因子2样蛋白2(NFE2L2)ELISA试剂盒 Human NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like 2) ELISA Kit

XY-EL-H1565c 人高密度脂蛋白(HDL)ELISA试剂盒 Human HDL (High Density Lipoprotein) ELISA Kit

XY-EL-H1566c 人抗脱氧核糖核蛋白抗体(DNP-Ab)ELISA试剂盒 Human DNP-Ab (Deoxyribonucleic Protein Antibody) ELISA Kit

XY-EL-H1567c 人乙型肝炎病毒表面抗原(HBsAg)ELISA试剂盒 Human HBsAg (Hepatitis B surface antigen) ELISA Kit 

XY-EL-H1571c 人基质金属蛋白酶原10 (Pro-MMP-10)ELISA试剂盒 Human Pro-MMP-10 (Pro-Matrix Metalloproteinase 10) ELISA Kit

XY-EL-H1572c 人基质金属蛋白酶原13 (Pro-MMP-13)ELISA试剂盒 Human Pro-MMP-13 (Pro-Matrix Metalloproteinase 13) ELISA Kit

XY-EL-H1573c 人信号调节蛋白α(SIRPA)ELISA试剂盒 Human SIRPA (Signal Regulatory Protein Alpha) ELISA Kit

XY-EL-H1574c 人血浆五聚蛋白3 (PTX 3/TSG-14)ELISA试剂盒 Human PTX 3/TSG-14 (Pentraxin 3, Long) ELISA Kit

XY-EL-H1575c 人血小板衍生生长因子AA(PDGF-AA)ELISA试剂盒 Human PDGF-AA (Platelet Derived Growth Factor-AA) ELISA Kit

XY-EL-H1576c 人血小板衍生生长因子AB(PDGF-AB)ELISA试剂盒 Human PDGF-AB (Platelet Derived Growth Factor AB) ELISA Kit 

XY-EL-H1577c 人血小板衍生生长因子BB(PDGF-BB)ELISA试剂盒 Human PDGF-BB (Platelet Derived Growth Factor-BB) ELISA Kit 

XY-EL-H1579c 人前蛋白转化酶枯草溶菌素9(PCSK9)ELISA试剂盒 Human PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) ELISA Kit

XY-EL-H1581c 人视黄醇结合蛋白4(RBP4)ELISA试剂盒 Human RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit

XY-EL-H1582c 人松弛肽2(RLN2)ELISA试剂盒 Human RLN2 (Relaxin-2) ELISA Kit

XY-EL-H1583c 人视黄醇结合蛋白1(RBP1)ELISA试剂盒 Human RBP1 (Retinol Binding Protein 1, Cellular) ELISA Kit

XY-EL-H1584c 人生存素(Surv)ELISA试剂盒 Human Surv (Survivin) ELISA Kit

XY-EL-H1585c 人视黄醇结合蛋白3(RBP3)ELISA试剂盒 Human RBP3 (Retinol Binding Protein 3, Interstitial) ELISA Kit

XY-EL-H1586c 人转化生长因子α(TGF-α)ELISA试剂盒 Human TGF-α (Transforming Growth Factor α) ELISA Kit

XY-EL-H1587c 人转化生长因子β2(TGF-β2)ELISA试剂盒 Human TGF-β2 (Transforming Growth Factor β2) ELISA Kit

XY-EL-H1588c 人血小板生成素(TPO)ELISA试剂盒 Human TPO (Thrombopoietin) ELISA Kit 

XY-EL-H1589c 人血小板反应蛋白1(TSP-1)ELISA试剂盒 Human TSP-1 (Thrombospondin-1) ELISA Kit

XY-EL-H1590c 人血小板反应蛋白2(TSP-2)ELISA试剂盒 Human TSP-2 (Thrombospondin-2) ELISA Kit

XY-EL-H1592c 人白介素7受体(IL7R)ELISA试剂盒 Human IL7R (Interleukin 7 Receptor) ELISA Kit

XY-EL-H1593c 人肿瘤坏死因子相关凋亡诱导配体(TRAIL/TNFSF10)ELISA试剂盒 Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit

XY-EL-H1594c 人破骨细胞分化因子(ODF)ELISA试剂盒 Human ODF (Osteoclast Differentiation Factor) ELISA Kit

XY-EL-H1595c 人磷脂酶D1(PLD1)ELISA试剂盒 Human PLD1 (Phospholipase D1) ELISA Kit

XY-EL-H1596c 人可溶性髓系细胞触发受体-1(sTREM-1)ELISA试剂盒 Human sTREM-1 (soluble Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit

XY-EL-H1597c 人髓系细胞触发受体-1(TREM-1)ELISA试剂盒 Human TREM-1 (Triggering Receptor Expressed on Myeloid Cells-1) ELISA Kit

XY-EL-H1598c 人胸腺基质淋巴细胞生成素(TSLP)ELISA试剂盒 Human TSLP (Thymic Stromal Lymphopoietin ) ELISA Kit 

XY-EL-H1599c 人尿激酶型纤溶酶原激活因子受体(PLAUR/uPAR)ELISA试剂盒 Human PLAUR/uPAR (Plasminogen Activator, Urokinase Receptor) ELISA Kit

XY-EL-H1600c 人血管内皮细胞生长因子C(VEGF-C)ELISA试剂盒 Human VEGF-C (Vascular Endothelial Growth Factor C) ELISA Kit 

XY-EL-H1601c 人血管内皮细胞生长因子D(VEGF-D)ELISA试剂盒 Human VEGF-D (Vascular Endothelial Growth Factor D) ELISA Kit 

XY-EL-H1603c 人血管内皮生长因子受体2(VEGFR-2/KDR)ELISA试剂盒 Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit

XY-EL-H1604c 人维生素D结合蛋白(DBP)ELISA试剂盒 Human DBP (Vitamin D Binding Protein) ELISA Kit 

XY-EL-H1605c 人胰岛素(INS)ELISA试剂盒 Human INS (Insulin) ELISA Kit