人组织多肽抗原(TPA)检测试剂盒

人组织多肽抗原(TPA)检测试剂盒实验操作常见问题分析

标本及采集、贮运因素：严重溶血 ，以 HRP 为标记的ELISA 测定中，残留在孔内的血红蛋白具有过氧化物酶样活性，催化底物显色造成假阳性； 混有红细胞的血清 易沉淀或附着在聚乙烯孔内不易洗净；如有 细菌污染 ，菌体中可能含有内源性HRP，也会产生假阳性反应； 标本凝固不全 ，有时为了争取时间快速检测，常在血液还未开始凝固时即强行离心分离血清，使血清中仍残留部分纤维蛋白原，在ELISA测定过程中可以形成肉眼可见的纤维蛋白块，易造成假阳性结果； 采血试管洗涤不彻底 、反复使用易交叉污染； 塑料试管能吸附抗原物质 ，样本久置在塑料管内会使样本内抗原含量下降造成假阴性。
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xyF644Hu人磷酸二酯酶4D(PDE4D)ELISA试剂盒ELISA Kit for Phosphodiesterase 4D, cAMP Specific (PDE4D) 

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xyD226Hu人胃脂酶(LIPF)ELISA试剂盒ELISA Kit for Lipase, Gastric (LIPF) 

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xyC996Hu人甘氨酰tRNA合成酶(GARS)ELISA试剂盒ELISA Kit for Glycyl tRNA Synthetase (GARS) 

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