缺氧诱导因子2α 抗体

缺氧诱导因子2α 抗体产品标准

浓缩液(1mg/1ml) 亲和纯化抗体

产品应用

WB=1:100-500 Elisa =1:200-1000 IP=1:20-100 IHC=1:100-500    （石蜡切片需做抗原修复）

缺氧诱导因子2α 抗体产品说明

   HIF-2α也是机体对缺氧适应性调节中的重要调控蛋白.有学者认为：HIF-2α对肿瘤的能量代谢和新血管生成有促进作用,微血管的形成可抑制肿瘤细胞的凋亡,从而促进肿瘤的恶性发展。

    Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Also known as Hypoxia-inducible factor -2α; Hypoxia-inducible factor 2 alpha; HIF-2 alpha; Endothelial PAS domain-containing protein 1; EPAS-1; HIF-1 alpha-like factor; MHLF; HIF-related factor; HRF; MOP2; Hif2a.
    [SUBUNIT] Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBPB.
    [SUBCELLULAR LOCATION] Nucleus (Potential).
    [TISSUE SPECIFICITY] Expressed in most tissues, with highest levels in lung, followed by heart, kidney, brain and liver. Predominantly expressed in endothelial cells. Also found in smooth muscle cells of the uterus, neurons, and brown adipose tissue. High expression in embryonic choroid plexus and kidney glomeruli.
    [DEVELOPMENTAL STAGE] In day 11 embryo, expression is almost exclusively seen in endothelial cells of the intersegmental blood vessels separating the somites, the atrial and ventricular chambers of the heart, and the dorsal aorta. High expression also occurs in extraembryonic membranes. In the developing brain of day 13 embryo, endothelial cells of the highly vascularized choroid plexus contain high levels of EPAS1.
    [PTM] In normoxia, is probably hydroxylated on Pro-405 and Pro-530 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity).
    [PTM] In normoxia, is hydroxylated on Asn-851 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
[    PTM] Phosphorylated on multiple sites in the CTAD.
    [PTM] The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
    [SIMILARITY] Contains 1 basic helix-loop-helix (bHLH) domain.
    [SIMILARITY] Contains 1 PAC (PAS-associated C-terminal) domain.
    [SIMILARITY] Contains 2 PAS (PER-ARNT-SIM) domains

缺氧诱导因子2α 抗体

hz-0818P MrpL28 (mitochondrial ribosomal protein L28)  线粒体核糖体蛋白L28(抗原)

hz-0836P MLH1(Mutl homolog l gene)  错配修复蛋白1抗原

hz-1022P MUC5AC/Mucin 5AC(Gastric Mucin M1)  胃粘液素抗原

hz-0963P MTNR1B/MTNR-1B(Melatonin receptor 1B)  褪黑素受体1B抗原

hz-1445P MLN(Motilin)peptide  胃动素多肽

hz-1992P mTOR(Mammalian target of rapamycin)  雷帕霉素靶蛋白

hz-1018P Muc-1/CD227  粘蛋白-1/上皮膜

hz-1047P MyD88 (myeloid differential protein-88)  髓样分化蛋白抗原

hz-2032P PMP2/FABP8(peripheral myelin protein 2)  周围神经髓鞘蛋白2抗原

hz-2166P MYSM1(Myb-like, SWIRM and MPN domain-containing protein 1)  MYSM1抗原

hz-1267P MYOZ/MYOZ1(Myozenin)human、ret、mouse  MYOZ抗原

hz-1049P CHRNA7(Nicotinic-Acetylcholine receptor alpha 7)  烟碱型乙酰胆碱受体α7(多肽)

hz-0829P Nanog fragment  干细胞关键蛋白(多肽)

hz-0903P Natrexone/BSA:Natrexone/KLH  纳曲酮偶联牛血清白蛋白：纳曲酮偶联血蓝蛋白

hz-1052P NAP1 (nucleosome asshzbly protein 1)  核小体组装蛋白1（多肽）

hz-1172P N-cadherin  N-钙粘附分子抗原

hz-1519P E-cadherin  E-钙粘附分子抗原

hz-0513P Nephrin Protein  去氧肾上腺素

hz-1055P Neurocan  神经粘蛋白抗原

hz-1366P NF2/merlin(neurofibromatosis type 2)  2型神经纤维瘤抗原

hz-1417P NFATc1  活化T细胞核因子1蛋白

hz-0982P phospho-NFKB p65 (pSer536) peptide  磷酸化细胞核因子/磷酸化k基因结合核因子抗原

hz-1194P NF-KapBp50(p50 NF-kappa B;p50NFKB)  细胞核因子50/κ基因结合核因子50抗原

hz-0922P NGN3(neurogenin 3; Neurog3)  神经元素3抗原

hz-1065P NGX6 (nasopharyngeal carcinoma/NPC associated gene 6)  鼻咽癌细胞相关基因6（多肽）

hz-2012P NIT2 (nitrilase family, mhzber 2)  NIT2蛋白抗原

hz-0069P NKA(Neurokinin A)  神经激肽A抗原

hz-2411P NKG2A/CD159a/KLRC1  NK细胞受体2A/自然杀伤细胞活化性受体2A抗原

hz-0938P NKG2D(natural killer cell group 2D)  NK细胞受体/自然杀伤细胞活化性受体

hz-0688P Nm23(NDP Kinase A,NDPKA)  肿瘤转移抑制基因抗原

hz-1066P Nm23-H1 (nucleoside dIPhosphatase kinase A)  肿瘤抑制基因抗原

hz-1315P Nogo-B/A  轴索过度生长抑制因子-B/A抗原

hz-2072P NOS-2 peptide  一氧化氮合成酶-2多肽抗原

hz-1335P Notch1/MOTC(Neurogenic locus notch homolog protein 1)  跨膜受体蛋白Notch-1抗原