凝固第XII因子/抗血友病因子抗体

英文名称    Factor alpha XIIa    

中文名称    凝固第XII因子/抗血友病因子抗体    

别    名    Coagulation factor XII; coagulation factor XIIa heavy chain; Coagulation factor XIIa light chain; F12; FA12_HUMAN; Factor XII; HAE3; HAEX; HAF; Hageman factor.    

说 明 书    0.2ml      

研究领域    心血管  免疫学      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Cow, Horse, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 凝固第XII因子/抗血友病因子抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    66kDa    

细胞定位    细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human Factor alpha XIIa    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

凝固第XII因子/抗血友病因子抗体产品介绍    background:

This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008]

Function:
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.

Subcellular Location:
Secreted.

Post-translational modifications:
Factor XII is activated by kallikrein in alpha-factor XIIa, which is then further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of the NH2-terminal heavy chain (Coagulation factor XIIa heavy chain) and the COOH-terminal light chain (Coagulation factor XIIa light chain), connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, a light chain (Beta-factor XIIa part 2), corresponding to the COOH-terminal light chain (Coagulation factor XIIa light chain) and a nonapeptide (Beta-factor XIIa part 1).
O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.

DISEASE:
Defects in F12 are the cause of factor XII deficiency (FA12D) [MIM:234000]; also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection). Defects in F12 are the cause of hereditary angioedema type 3 (HAE3) 
[MIM:610618]; also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g., during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal.

Similarity:
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 fibronectin type-I domain.
Contains 1 fibronectin type-II domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain.

Gene ID:
2161

Database links:

Entrez Gene: 2161 Human

Omim: 610619 Human

SwissProt: P00748 Human

Unigene: 1321 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

凝固第XII因子/抗血友病因子抗体

hze-0302R Rabbit Anti-HRP/HRP  辣根过氧化物酶标记兔抗HRP

hze-0360R Rabbit Anti-human IgA/HRP  辣根过氧化物酶标记兔抗人IgA

hze-0437P Streptavidin/HRP  辣根过氧化物酶标记链霉亲和素

hze-0362P Protein A/HRP  辣根过氧化物酶标记的蛋白A

hze-0292P BSA/HRP   辣根过氧化物酶标记牛血清白蛋白

hze-0361P rec Protein G/HRP  辣根过氧化物酶标记的重组蛋白G

hz-0855P-HRP Insulin/HRP  辣根过氧化物酶标记人胰岛素蛋白

hz-0311P-AP Biotin/AP  碱性磷酸酶标记生物素

hz-0312P-AP Avidin/AP  碱性磷酸酶标记亲合素

hz-0437P-AP Streptavidin/AP  碱性磷酸酶标记链霉亲和素

hz-0360G-AP Goat Anti-human IgA/AP  碱性磷酸酶标记羊抗人IgA

hz-0360R-AP Rabbit Anti-human IgA/AP  碱性磷酸酶标记兔抗人IgA

hz-0362P-AP Protein A/AP  碱性磷酸酶标记的蛋白A

hz-0292P-AP BSA/AP  碱性磷酸酶标记标记牛血清白蛋白

hz-0361P-AP rec Protein G/AP  碱性磷酸酶标记的重组蛋白G

hzb-0360G Goat Anti-human IgA/Biotin  生物素化羊抗人IgA

hza-0297R Rabbit Anti-human IgG/Streptavidin  链霉亲和素标记兔抗人IgG

hzb-0295P Rabbit IgG/Biotin  生物素化兔IgG

hzb-0297P human IgG/Biotin  生物素化人IgG

hzb-0296P mouse IgG/Biotin  生物素化小鼠IgG

hzF-0292P BSA/FITC  荧光素标记牛血清白蛋白

hzF-0366R Rb Anti-FITC/FITC  荧光素标记兔抗FITC

hzF-0360G Goat Anti-human IgA/FITC  荧光素标记羊抗人IgA

hzR-0292P BSA/RBITC  罗丹明标记牛血清白蛋白

hzR-0437P Streptavidin/RBITC  红色荧光素罗丹明标记链霉亲和素

hzR-0295P Rabbit IgG/RBITC  罗丹明标记兔IgG(细胞流式同型对照)

hzR-0296P Mouse IgG/RBITC  罗丹明标记小鼠IgG(细胞流式同型对照)

hzR-0294P Goat IgG/RBITC  罗丹明标记羊IgG(细胞流式同型对照)

hzR-0293P Rat IgG/RBITC  罗丹明标记大鼠IgG(细胞流式同型对照)

hzF-0312P Avidin/FITC  荧光素标记亲合素

hzF-0437P Streptavidin/FITC   荧光素标记链霉亲和素

hzF-0361P rec Protein G/FITC  荧光素标记重组纯化蛋白G

hzF-0362P Protein A/FITC  FITC标记重组金黄色葡萄球菌蛋白A

hzR-0366R Rb Anti-FITC/RBITC  罗丹明标记兔抗FITC