英文名称 eEF1A1
中文名称 翻译延伸因子EF-1a抗体
别 名 Elongation Factor 1, Alpha; EF1 Alpha; eukaryotic translation elongation factor 1 alpha 1; CCS3; eEF1A-1; EF-Tu; EF1A; Elongation factor Tu; GRAF-1EF; PTI1; elongation factor 1-alpha 1; EEF-1; EEF1A; Eef1a2; Eef1a2l1; EF1A; MGC125172; SI; Eef1a1; EF1A1_HUMAN.
说 明 书 0.1ml 0.2ml
研究领域 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 表观遗传学
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 翻译延伸因子EF-1a抗体(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 50kDa
细胞定位 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human EF1 alpha
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
翻译延伸因子EF-1a抗体产品介绍 background:
eEF1A1 is an isoform of the alpha subunit of the elongation factor 1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. It promotes the GTP dependent binding of aminoacyl tRNA to the A site of ribosomes during protein biosynthesis. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome (referenced from entrez gene and swissprot).
Function:
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle
Post-translational modifications:
ISGylated.
Similarity:
Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
Gene ID:
1915
Database links:
Entrez Gene: 1915 Human
Entrez Gene: 13627 Mouse
Entrez Gene: 171361 Rat
Omim: 130590 Human
SwissProt: P68104 Human
SwissProt: P10126 Mouse
SwissProt: P62630 Rat
Unigene: 520703 Human
Unigene: 535192 Human
Unigene: 586423 Human
Unigene: 695681 Human
Unigene: 713109 Human
Unigene: 138471 Mouse
Unigene: 335315 Mouse
Unigene: 360075 Mouse
Unigene: 391071 Mouse
Unigene: 468942 Mouse
Unigene: 144660 Rat
Unigene: 965 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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