圻明试剂 干扰素γ(IFNg)活性蛋白

Source: Eukaryotic expression. Host: 293F cell

Residues: Gln24~Gln166

Tags: N-terminal His-tag

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and Proclin300. Predicted isoelectric point: 9.7

Predicted Molecular Mass: 18.4kDa

Accurate Molecular Mass: 22&25kDa as determined by SDS-PAGE reducing

conditions. Applications: Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0

mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month

[ ACTIVITY ]

IFN-γ is a dimerized soluble cytokine that is the only member of the type II class

of interferons.The importance of IFNγ in the immune system stems in part from

its ability to inhibit viral replication directly, and most importantly from its

immunostimulatory and immunomodulatory effects. As reported, IFN γ is an

important activator of human monocytic THP1 cells. Therefore,THP-1 cells were

incubated in RPMI 1640 with various concentration of IFN-γ, then cells were

observed by inverted microscope everyday.After stimulated with IFN-γ(5ng/ml)

for 5 days, morphological changes occured in THP1 cells which displayed the

shape of fusiform or polygon and were more likely to adhere.

mYF9405 骨涎蛋白(BSP)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9406 凝血因子ⅩⅢA1肽(F13A1)重组蛋白 Rattus norvegicus (Rat，大鼠)

mYF9407 纤维蛋白原α(FGa)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9408 硫氧还蛋白结合蛋白2(TBP2)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9409 VGF神经生长因子诱导蛋白(VGF)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9410 葡萄糖转运蛋白1(GLUT1)重组蛋白 Rattus norvegicus (Rat，大鼠)

mYF9411 胃泌素(GT)重组蛋白 Cavia (Guinea pig ，豚鼠)

mYF9412 胃泌素(GT)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9413 ATP结合盒转运蛋白A1(ABCA1)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9414 心型脂肪酸结合蛋白(FABP3)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9415 乙酰辅酶A羧化酶α(ACACa)重组蛋白 Homo sapiens (Human，人)

mYF9416 乙酰辅酶A羧化酶α(ACACa)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9417 乙酰辅酶A羧化酶α(ACACa)重组蛋白 Rattus norvegicus (Rat，大鼠)

mYF9418 野鼠色基因相关蛋白(AGRP)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9419 补体成分4b(C4b)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9420 钙调结合蛋白(CALD)重组蛋白 Homo sapiens (Human，人)

mYF9421 钙调结合蛋白(CALD)重组蛋白 Rattus norvegicus (Rat，大鼠)

mYF9422 鸟氨酸脱羧酶(ODC)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9423 鸟氨酸脱羧酶(ODC)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9424 鸟氨酸脱羧酶(ODC)重组蛋白 Rattus norvegicus (Rat，大鼠)

mYF9425 β-1,3-葡糖醛酸基移酶1(b3GAT1)重组蛋白 Homo sapiens (Human，人)

mYF9426 弹性蛋白(ELN)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9427 胰脂肪酶(PL)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9428 胰淀粉酶α2(AMY2)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9429 胰淀粉酶α2(AMY2)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9430 软骨糖蛋白39(GP39)重组蛋白 Rattus norvegicus (Rat，大鼠)

mYF9431 基质Gla蛋白(MGP)重组蛋白 Mus musculus (Mouse，小鼠)

mYF9432 肝型脂肪酸结合蛋白(FABP1)重组蛋白 Rattus norvegicus (Rat，大鼠)