草酰琥珀酸脱羧酶抗体

以下为您提供的是草酰琥珀酸脱羧酶抗体的说明书,了解更多关于草酰琥珀酸脱羧酶抗体的英文名称,草酰琥珀酸脱羧酶抗体别名,草酰琥珀酸脱羧酶抗体规格,请看以下详细介绍.

英文名称  Anti-IDH2

中文名称  草酰琥珀酸脱羧酶抗体

别    名  mitochondrial; ICD-M; IDH; IDH2; IDHP_HUMAN; IDP; Isocitrate dehydrogenase [NADP]; Isocitrate dehydrogenase [NADP], mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase.

浓    度  1mg/1ml

规 格  0.1ml/100μg  0.2ml/200μg    

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Dog, Horse

草酰琥珀酸脱羧酶抗体的多样性：

抗体的异质性。抗体的组成极为复杂，是由成千上万、多种多样的免疫球蛋白（Ig）分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上，既相似，又有差别。由于有差别，它们的电泳活性就有很大的变化。

因为抗体具有与抗原决定簇相对应的结合部位（抗原结合簇），所以抗体与抗原的结合具有特异性。另一方面，抗体本身是一种蛋白质，具有本身的氨基酸组成、排列和立体结构，对异种动物来说，它又是抗原。各类Ig都具有可用血清学方法检出的抗原特异性，它们表现出不同的血清学类型。

草酰琥珀酸脱羧酶抗体的相关产品:

Anti-TLR5/CD285   Toll样受体5抗体 0.1ml

Anti-TM   血栓调节蛋白抗体 0.2ml

Anti-TmR2   胶质细胞系源性神经营养因子受体β抗体 0.2ml

anti-Tn-C   腱糖蛋白-C抗体 0.2ml

Anti-TNF-α   肿瘤坏死因子-α抗体 0.1ml

产品类型  一抗    

研究领域  肿瘤 细胞生物 免疫学 染色质和核信号 转录调节因子 激酶和磷酸酶 新陈代谢  

蛋白分子量  predicted molecular weight: 51kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human IDH2

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 IDH2 (isocitrate dehydrogenase 2 (NADP+), mitochondrial), also designated NADP+-specific ICDH; isocitrate dehydrogenase, mitochondrial; and oxalosuccinate decarboxylase, is a 452 amino acid enzyme encoded by the human gene IDH2. IDH2 belongs to the isocitrate and isopropylmalate dehydrogenases family and contains two nucleotide binding regions. IDH2 is involved in the reduction of NADP+ to NADPH and maintains the supply of glutathione (GSH) in mitochondria. It is believed to play a role in intermediary metabolism and energy production. IDH2 also tightly associates with the pyruvate dehydrogenase complex. IDH2 is found in the mitochondrion as a homodimer and can bind one magnesium or manganese ion per subunit.

Function : Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.

Subunit : Homodimer.

Subcellular Location : Cytoplasm. Peroxisome.

DISEASE : Defects in IDH1 are involved in the development of glioma (GLM) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Mutations affecting Arg-132 are tissue-specific, and suggest that this residue plays a unique role in the development of high-grade gliomas. Mutations of Arg-132 to Cys, His, Leu or Ser abolish magnesium binding and abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. Elevated levels of R(-)-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.

草酰琥珀酸脱羧酶抗体Similarity : Belongs to the isocitrate and isopropylmalate dehydrogenases family.

IDH2又称异柠檬酸脱氢酶，他有有两种类型的酶：以NAD为辅酶的酶（EC1．1．1．41）和NADP的酶（EC1．1．1．42），两者催化同一反应。在胞浆中以NADP为辅酶，在线粒体内膜以NAD为辅酶。 　　 IDH2在三羧酸循环中正常起作用的是NAD为辅酶的酶，他是提供为ADP激活的变构酶。