磷酸化干扰素调节因子3

公司磷酸化干扰素调节因子3具有高纯度、高效价、高特异性的特点，仅用于科学研究，不可用于临床诊断及药物治疗，可以应用于多种实验。CST、Santa抗体品牌。

英文名称  Anti-Phospho-IRF3 (Ser396)

中文名称  磷酸化干扰素调节因子3

别    名  IRF3 (phospho S396); IRF3 (phospho Ser396); p-IRF3 (Ser396); p-IRF3 (S396); Interferon regulatory factor 3; IRF 3; IRF-3; IRF3; IRF3_HUMAN; MGC94729.

浓    度  1mg/1ml

规 格  0.1ml/100μg
磷酸化干扰素调节因子3 的相关产品:

Mouse Anti-YERSINIA PESTIS Monoclonal Antibody   鼠抗鼠疫F1蛋白/鼠疫耶尔森氏菌荚膜抗原F1单克隆抗体 0.1ml

Shiga-like toxin IIe variant subunit A[Escherichia coli]   抗猪水肿病大肠杆菌志贺样毒素Ⅱ型突变体抗体 0.2ml

SLC34A2/NaPi-2b   磷酸钠协同转运蛋白抗体 0.1ml      

磷酸化干扰素调节因子3抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Sheep

产品类型  一抗  磷酸化抗体  

研究领域  肿瘤 免疫学 信号转导 细胞凋亡 转录调节因子  

蛋白分子量  predicted molecular weight: 47kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated synthesised phosphopeptide derived from human IRF3 around the phosphorylation site of Ser396

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].

Function : Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Subunit : Monomer. Homodimer; phosphorylation-induced. Heterodimer with IRF7. Interacts with CREBBP. May interact with MAVS. Interacts with IKBKE and TBK1. Interacts with TICAM1 and TICAM2. Interacts with rotavirus A NSP1 (via C-terminus); this interaction leads to the proteasome-dependent degradation of IRF3. Interacts with RBCK1. Interacts with TRIM21. Interacts with HERC5.

Subcellular Location : Cytoplasm. Nucleus. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.

Tissue Specificity : Expressed constitutively in a variety of tissues.

Post-translational modifications : Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.

Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.

磷酸化干扰素调节因子3ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.

Similarity : Belongs to the IRF family.

Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

Database links : UniProtKB/Swiss-Prot: Q14653

干扰素调节因子家族是一大类对干扰素起调控作用的转录因子的统称。 一般认为干扰素调节因子(IRF)通过调节干扰素的表达而行使其抗病毒、应激、免疫调节功能。近年来，研究人员发现IRF在细胞凋亡、细胞周期、细胞分化、肿瘤发生中也起着重要的调节作用。

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