磷酸化白介素-1受体相关激酶1抗体

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英文名称  Anti-Phospho-IRAK1 (Ser376)

中文名称  磷酸化白介素-1受体相关激酶1抗体

别    名  IRAK1 (Phospho-Ser376); IRAK1 (Phospho-S376); IRAK1 (p-Ser376); Il1rak; Il1rak; Interleukin 1 receptor associated kinase 1; Interleukin 1 receptor associated kinase 1; Interleukin 1 receptor associated kinase 2; Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; IRAK1; IRAK1; IRAK1_HUMAN; IRAK2; IRAK2; mPLK; mPLK; Pelle; Pelle; Pelle homolog; Pelle-like protein kinase; Plpk.

浓    度  1mg/1ml

规 格  0.1ml/100μg    

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Mouse, Rat, Dog, Rabbit

磷酸化白介素-1受体相关激酶1抗体的多样性：

抗体的异质性。抗体的组成极为复杂，是由成千上万、多种多样的免疫球蛋白（Ig）分子所组成。这些Ig分子在形状、大小、结构以及氨基酸的组成和排列上，既相似，又有差别。由于有差别，它们的电泳活性就有很大的变化。

因为抗体具有与抗原决定簇相对应的结合部位（抗原结合簇），所以抗体与抗原的结合具有特异性。另一方面，抗体本身是一种蛋白质，具有本身的氨基酸组成、排列和立体结构，对异种动物来说，它又是抗原。各类Ig都具有可用血清学方法检出的抗原特异性，它们表现出不同的血清学类型。

磷酸化白介素-1受体相关激酶1抗体的相关产品:

Anti-TGF-α   转移生长因子–α抗体 0.1ml

Anti-TGF-β1   转移生长因子–β1抗体 0.1ml

Anti-TGF-β2   转移生长因子–β2抗体 0.1ml

Anti-TGF-β3   转移生长因子–β3抗体 0.1ml

产品类型  一抗  磷酸化抗体  

研究领域  肿瘤 细胞生物 免疫学 细胞凋亡 激酶和磷酸酶  

蛋白分子量  predicted molecular weight: 78kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated synthesised phosphopeptide derived from human IRAK1 around the phosphorylation site of Ser376 [QS(p-S)TV]

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500

（石蜡切片需做抗原修复）

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍 IRAK or Interleukin-1 Receptor-associated Kinase 1, is one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This protein is partially responsible for IL1-induced upregulation of the ubiquitous transcription factor NF-kappa B.

Function : Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Subunit : Homodimer. Interacts with TOLLIP; this interaction occurs in the cytosol prior to receptor activation. Interacts with MYD88; this interaction recruits IRAK1 to the stimulated receptor complex. Interacts with IL1RL1. Interacts with IRAK1BP1. Associates with TRAF6, PELI1 and IRAK4; this complex recruits MAP3K7/TAK1, TAB1 and TAB2 to mediate NF-kappa-B activation. Interacts (when polyubiquitinated) with IKBKG/NEMO.

Subcellular Location : Cytoplasm. Nucleus. Note=Translocates to the nucleus when sumoylated.

Tissue Specificity : Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Post-translational modifications : Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity.

Polyubiquitinated after cell stimulation with IL-1-beta by PELI1, PELI2 and PELI3. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. 磷酸化白介素-1受体相关激酶1抗体nuclear translocation.

Similarity : Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Contains 1 death domain.

Contains 1 protein kinase domain.

Database links : UniProtKB/Swiss-Prot: P51617.2