HERC2 E3泛素蛋白连接酶抗体

HERC2 E3泛素蛋白连接酶抗体

〖别名〗HECT domain and RCC1 like domain containing protein 2; Hect domain and RLD 2; jdf2; p528; SHEP1; HERC2_HUMAN. 

〖浓度〗1mg/1ml 

〖规格〗0.2ml/200μg     

〖抗体来源〗Rabbit  

〖克隆类型〗polyclonal 

〖交叉反应〗Human

〖产品类型〗一抗    

〖研究领域〗细胞生物  

〖蛋白分子量〗predicted molecular weight: 527kDa 

〖性状〗Lyophilized or Liquid 

〖免疫原〗KLH conjugated synthetic peptide derived from human HERC2 

〖亚型〗IgG 

〖纯化方法〗affinity purified by Protein A 

〖储存液〗Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 

〖产品应用〗WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

〖保存条件〗Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

〖产品介绍〗HERC2 is a belongs to the HERC gene family that encodes a group of unusually large proteins, which contain multiple structural domains. All members have at least 1 copy of an N-terminal region showing homology to the cell cycle regulator RCC1 and a C-terminal HECT (homologous to E6-AP C terminus) domain found in a number of E3 ubiquitin protein ligases. Genetic variations in this gene are associated with skin/hair/eye pigmentation variability. Multiple pseudogenes of this gene are located on chromosomes 15 and 16.

Function : E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity.

Subunit : Interacts (when phosphorylated at Thr-4827 and sumoylated) with RNF8 (via FHA domain); this interaction increases after ionizing radiation (IR) treatment. Interacts with XPA. Interacts with NEURL4. Via its interaction with NEURL4, may indirectly interact with CCP110 and CEP97.

Subcellular Location : Cytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole. Nucleus. Note=Recruited to sites of DNA damage in response to ionizing radiation (IR) via its interaction with RNF8. May loose association with centrosomes during mitosis.

Post-translational modifications : Phosphorylation at Thr-4827 is required for interaction with RNF8.

Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs), promoting the interaction with RNF8.

Similarity : Contains 1 cytochrome b5 heme-binding domain.

Contains 1 DOC domain.

Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.

Contains 1 MIB/HERC2 domain.

Contains 19 RCC1 repeats.

Contains 6 WD repeats.

Contains 1 ZZ-type zinc finger.

Database links : UniProtKB/Swiss-Prot: O95714.2

做过实验的亲们都知道反应规律，仔细观察我们会发现当抗原第一次进入机体时,需经一定的潜伏期才能产生抗体,且抗体产生的量也不多,在体内维持的时间也较短。当相同抗原第二次进入机体后,开始时,由于原有抗体中的一部分与再次进入的抗原结合,可使原有抗体量略为降低。随后,抗体效价迅速大量增加,可比初次反应产生的多几倍到几十倍,在体内留存的时间亦较长。因此得出由抗原刺激机体产生的抗体,经过一定时间后可逐渐消失。此时若再次接触抗原,可使已消失的抗体快速上升。如再次刺激机体的抗原与初次相同,则称为特异性回忆反应；若与初次反应不同,则称为非特异性回忆反应。  

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