BMPs (Bone Morphogenetic Proteins) belong to the TGF-β superfamily of
structurally related signaling proteins. BMP-2 is a potent
osteoinductive cytokine, capable of inducing bone and cartilage
formation in association with an osteoconductive carrier such as
collagen and synthetic hydroxyapatite. In addition to its osteogenic
activity, BMP-2 appears to play an important role in cardiac
morphogenesis, and is expressed in a variety of other tissues, including
lung, liver, spleen, prostate, ovary, and small intestine. The
functional form of BMP-2 is a 26 kDa protein composed of two identical
114 amino acid polypeptide chains (monomers) linked by a single
disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part
of a precursor polypeptide, which also contains a 23 amino acid signal
sequence for secretion, and a 259 amino acid propeptide. After
dimerization of this precursor, the covalent bonds between the
propeptide (which is also a disulfide-linked homodimer) and the mature
BMP-2 ligand are cleaved by a furin-type protease. Recombinant
Human/Murine/Rat BMP-2 derived from CHO cells is a homodimeric
glycoprotein that consists of two 114 amino acid polypeptide chains
linked by a single disulfide bond. Due to glycosylation, CHO
cell-derived Human/Murine/Rat BMP-2 migrates at an apparent molecular
weight of approximately 28-29 kDa by SDS-PAGE analysis under
non-reducing conditions.
Source: CHO cells
Synonyms: Bone Morphogenetic Protein-2, BMP-2A
AA Sequence (monomer): QAKHKQRKRL KSSCKRHPLY VDFSDVGWND WIVAPPGYHA FYCHGECPFP LADHLNSTNH AIVQTLVNSV NSKIPKACCV PTELSAISML YLDENEKVVL KNYQDMVVEG CGCR
Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.
Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 40-100 ng/ml.
This product has been tested against the WHO standard. International Unit information can be found here.
Calculated Molecular Weight: 25.8 kDa
Accession Number: P12643
Gene ID: 650
Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)
crossreactivity:
Human, Mouse,
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