HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include the induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear, polypeptide-precursor glycoprotein containing 697 amino acid residues. Proteolytic processing of this precursor generates the biologically active heterodimeric form of HGF, which consists of two polypeptide chains (α-chain and β-chain) held together by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues. Recombinant Human HGF, sourced from HEK293 cells, is a 79.4 kDa polypeptide consisting of 695 amino acid residues. As a result of glycosylation, Recombinant Human HGF migrates with an apparent molecular mass of approximately 68-85 kDa by SDS-PAGE gel, under non-reducing conditions.
Source: HEK293 cells
Synonyms: Hepatocyte Growth Factor, Scatter Factor (SF), Hepatopoietin (HPTA)
AA Sequence:
alpha chain: KRRNTIHEFK KSAKTTLIKI DPALKIKTKK VNTADQCANR CTRNKGLPFT CKAFVFDKAR KQCLWFPFNS MSSGVKKEFG HEFDLYENKD YIRNCIIGKG RSYKGTVSIT KSGIKCQPWS SMIPHEHSFL PSSYRGKDLQ ENYCRNPRGE EGGPWCFTSN PEVRYEVCDI PQCSEVECMT CNGESYRGLM DHTESGKICQ RWDHQTPHRH KFLPERYPDK GFDDNYCRNP DGQPRPWCYT LDPHTRWEYC AIKTCADNTM NDTDVPLETT ECIQGQGEGY RGTVNTIWNG IPCQRWDSQY PHEHDMTPEN FKCKDLRENY CRNPDGSESP WCFTTDPNIR VGYCSQIPNC DMSHGQDCYR GNGKNYMGNL SQTRSGLTCS MWDKNMEDLH RHIFWEPDAS KLNENYCRNP DDDAHGPWCY TGNPLIPWDY CPISRCEGDT TPTIVNLDHP VISCAKTKQL R
beta chain: VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS
Purity: ≥ 95% by SDS-PAGE gel and HPLC analyses.
Biological Activity: Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The expected ED50 for this effect is 1.0-20.0 ng/ml.
Recombinant Human HGF (HEK293 derived) Biological Activity Graph
Calculated Molecular Weight: 79.4 kDa
Accession Number: P14210
Gene ID: 3082
Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)
Cross Reactivity Cited in References:
Human, Mouse
企业名称
上海远慕生物科技有限公司
企业信息已认证
企业类型
有限责任公司(自然人独资)
信用代码
91310120071207690P
成立日期
2013-06-19
注册资本
人民币300.0000万元整
经营范围
从事生物科技领域内的技术开发、技术咨询、技术服务、技术转让,实验室设备、化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品)、仪器仪表、五金交电、电子产品、机械设备、计算机、软件及辅助设备(除计算机信息系统安全专用产品)、电动自行车的批发、零售.(依法须经批准的项目,经相关部门批准后方可开展经营活动)
上海远慕生物科技有限公司
公司地址
上海浦东新区
客服电话