缓冲葡萄糖蛋白胨水培养基

我公司专业销售缓冲葡萄糖蛋白胨水培养基微生物培养基、细胞培养基、菌种培养基、进口培养基、国产培养基公司现货直销缓冲葡萄糖蛋白胨水培养基，培养基价格、培养基报价、培养基批发，欢迎有培养基需要的科研工作者来电咨询！
产品编号：FS1454

产品名称：缓冲葡萄糖蛋白胨水培养基

产品规格：250g/瓶

用途：用于细菌的 MR、VP 试验

缓冲葡萄糖蛋白胨水培养基使用方法：

（1）称取Pfizer 肠球菌选择性琼脂（PSE 琼脂）50g，加入蒸馏水或去离子水1 L，搅拌加热煮沸至完全溶解，分装三角瓶，121℃高压灭菌20min，备用。

（2）检样的稀释处理。

（3）选取3个合适的稀释级的样品1 mL，分别注入平皿，每个稀释级做2—3个平板，每皿加15—20 mL已冷至45℃左右的YPD培养基，加毕立即摇匀，待凝固后倒置于20—25℃培养箱中培养72h。

（4）观察结果。对于菌落微小点计困难的平板可延续培养到4—5d再行观察计数。

（5）计数：通常选择菌落数在30-100之间的稀释级的平板计数，取其平均数乘以稀释倍数报告。

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