动力-硝酸盐培养基（A 法）

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产品编号：FS1436

产品名称：动力-硝酸盐培养基（A 法）

产品规格：250g/瓶

用途：用于细菌动力试验

动力-硝酸盐培养基（A 法）使用方法：

（1）称取Pfizer 肠球菌选择性琼脂（PSE 琼脂）50g，加入蒸馏水或去离子水1 L，搅拌加热煮沸至完全溶解，分装三角瓶，121℃高压灭菌20min，备用。

（2）检样的稀释处理。

（3）选取3个合适的稀释级的样品1 mL，分别注入平皿，每个稀释级做2—3个平板，每皿加15—20 mL已冷至45℃左右的YPD培养基，加毕立即摇匀，待凝固后倒置于20—25℃培养箱中培养72h。

（4）观察结果。对于菌落微小点计困难的平板可延续培养到4—5d再行观察计数。

（5）计数：通常选择菌落数在30-100之间的稀释级的平板计数，取其平均数乘以稀释倍数报告。

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